East Tokyo Laboratory, Genesis Research Institute, Inc., 717-86 Futamata, Ichikawa, Chiba 272-0001, Japan.
Phys Chem Chem Phys. 2011 Jan 14;13(2):586-92. doi: 10.1039/c0cp00770f. Epub 2010 Nov 1.
We have developed a new method for selective decomposition of nucleic acids. The method utilizes a high temperature and pressure region (HTP region, hereafter) around a gold nanoparticle, which was generated when the gold nanoparticle was irradiated with a pulsed laser in aqueous solution. A probe DNA molecule whose sequence was complementary to a part of a target DNA molecule was bound to the gold nanoparticle surface. In a solution containing both the target and non-target DNA molecules, the gold nanoparticle selectively attached to the target DNA through hybridization of the probe DNA. When the gold nanoparticle was excited by a pulsed laser, the HTP region was generated in the close vicinity of the gold nanoparticle and then the target DNA molecules inside of this region were decomposed. The non-target DNA molecules having no part complementary to the probe DNA were scarcely decomposed by laser irradiation. When the gold nanoparticle was excited by an intense laser, the non-target DNA molecules were also decomposed, because some of them were located inside the inflated HTP region. We discussed the mechanism of the decomposition of the DNA molecules by the HTP region.
我们开发了一种新的选择性核酸分解方法。该方法利用了金纳米粒子在水溶液中被脉冲激光照射时产生的高温高压区域（HTP 区域，以下简称）。与靶 DNA 分子的一部分互补的探针 DNA 分子被结合到金纳米粒子表面。在含有靶 DNA 分子和非靶 DNA 分子的溶液中，金纳米粒子通过探针 DNA 的杂交选择性地与靶 DNA 结合。当金纳米粒子被脉冲激光激发时，在金纳米粒子的近旁产生 HTP 区域，然后该区域内的靶 DNA 分子被分解。没有与探针 DNA 互补部分的非靶 DNA 分子几乎不会被激光照射分解。当金纳米粒子被强激光激发时，非靶 DNA 分子也被分解，因为其中一些位于膨胀的 HTP 区域内。我们讨论了 HTP 区域分解 DNA 分子的机制。
