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人β-防御素在儿童慢性炎症性肠病中的表达。

Expression of human beta-defensins in children with chronic inflammatory bowel disease.

机构信息

Department of Paediatrics, HELIOS Klinikum Wuppertal, Germany.

出版信息

PLoS One. 2010 Oct 22;5(10):e15389. doi: 10.1371/journal.pone.0015389.

DOI:10.1371/journal.pone.0015389
PMID:21042595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2962650/
Abstract

BACKGROUND

Human beta-defensins (hBDs) are antimicrobial peptides known to play a major role in intestinal innate host defence. Altered mucosal expression of hBDs has been suggested to be implicated in chronic inflammatory bowel disease pathogenesis. However, little is known about expression of these peptides in children.

METHODS

Intestinal biopsies were obtained from the duodenum (n = 88), terminal ileum (n = 90) and ascending colon (n = 105) of children with Crohn's disease (n = 26), ulcerative colitis (n = 11) and healthy controls (n = 16). Quantitative real-time (RT) PCR was performed and absolute mRNA copy numbers analyzed for hBD1-3 as well as inflammatory cytokines IL-8 and TNF-alpha.

RESULTS

Significant induction of hBD2 and hBD3 was observed in the inflamed terminal ileum and ascending colon of IBD children. In the ascending colon induction of hBD2 was found to be significantly lower in children with Crohn's disease compared to ulcerative colitis. A strong correlation was found between inducible defensins hBD2 and 3 and the inflammatory cytokines IL-8 and TNF-alpha, both in the terminal ileum and ascending colon.

CONCLUSION

Our study demonstrates distinct changes in hBD expression throughout the intestinal tract of children with IBD, lending further support for their potential role in disease pathogenesis.

摘要

背景

人β-防御素（hBDs）是一种已知在肠道先天宿主防御中起主要作用的抗菌肽。hBDs 的粘膜表达改变被认为与慢性炎症性肠病的发病机制有关。然而，关于这些肽在儿童中的表达知之甚少。

方法

从克罗恩病（n=26）、溃疡性结肠炎（n=11）和健康对照儿童（n=16）的十二指肠（n=88）、末端回肠（n=90）和升结肠（n=105）获得肠活检。进行定量实时（RT）PCR，并分析 hBD1-3 以及炎症细胞因子 IL-8 和 TNF-α的绝对 mRNA 拷贝数。

结果

在 IBD 儿童的炎症末端回肠和升结肠中观察到 hBD2 和 hBD3 的显著诱导。在升结肠中，与溃疡性结肠炎相比，克罗恩病儿童的 hBD2 诱导明显较低。在末端回肠和升结肠中，诱导型防御素 hBD2 和 3 与炎症细胞因子 IL-8 和 TNF-α之间存在很强的相关性。

结论

我们的研究表明，IBD 儿童整个肠道的 hBD 表达发生了明显变化，进一步支持它们在疾病发病机制中的潜在作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/e5ae9a121455/pone.0015389.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/6534358d7bf6/pone.0015389.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/2c559a093a18/pone.0015389.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/3c7e1f0ab35c/pone.0015389.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/e5ae9a121455/pone.0015389.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/6534358d7bf6/pone.0015389.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/2c559a093a18/pone.0015389.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/3c7e1f0ab35c/pone.0015389.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d83/2962650/e5ae9a121455/pone.0015389.g004.jpg

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