Dunkel L, Alfthan H, Stenman U H, Perheentupa J
Children's Hospital, Helsinki, Finland.
J Clin Endocrinol Metab. 1990 Jan;70(1):107-14. doi: 10.1210/jcem-70-1-107.
To elucidate the role of the testis in the control of LH and FSH secretion before puberty, we examined pulsatile LH and FSH secretion in six prepubertal boys with primary testicular failure (two boys with masculine pseudohermaphroditism, two boys with the Klinefelter's syndrome, and two boys with anorchia) and eight normal prepubertal boys. Plasma LH and FSH levels were measured every 15 min for 6 h during the day and night with ultrasensitive (0.019 and 0.014 IU/L) time-resolved immunofluorometric assays. In all six hypogonadal boys the mean FSH level was above the range of the normal prepubertal boys, whereas the LH level was elevated in only one boy. All boys had LH and FSH pulses. The FSH pulse interval in the anorchid boys was shorter than that in the normal boys, but this was not observed in the other hypogonadal boys. The LH pulse interval in the anorchid and other hypogonadal boys was the same as that in the normal boys. The FSH pulse amplitudes were higher in the anorchid and other hypogonadal boys than in the normal boys, but the LH pulse amplitudes were higher only in the anorchid boys. We conclude that in prepuberty the testes have little effect on LH secretion, but that they are involved in the regulation of FSH levels. In primary testicular failure, the elevation of FSH levels is associated with an increase in FSH pulse amplitude and, in the absence of testicular steroids, possibly also with an increase in FSH pulse frequency.
为阐明青春期前睾丸在促黄体生成素（LH）和促卵泡生成素（FSH）分泌调控中的作用，我们检测了6名青春期前原发性睾丸功能衰竭男孩（2名男性假两性畸形男孩、2名克兰费尔特综合征男孩和2名无睾症男孩）以及8名正常青春期前男孩的LH和FSH脉冲式分泌情况。采用超灵敏（0.019和0.014 IU/L）时间分辨免疫荧光分析法，在白天和夜间每隔15分钟测量一次血浆LH和FSH水平，持续6小时。在所有6名性腺功能减退男孩中，平均FSH水平高于正常青春期前男孩的范围，而只有1名男孩的LH水平升高。所有男孩都有LH和FSH脉冲。无睾症男孩的FSH脉冲间隔比正常男孩短，但在其他性腺功能减退男孩中未观察到这种情况。无睾症和其他性腺功能减退男孩的LH脉冲间隔与正常男孩相同。无睾症和其他性腺功能减退男孩的FSH脉冲幅度高于正常男孩，但只有无睾症男孩的LH脉冲幅度较高。我们得出结论，青春期前睾丸对LH分泌影响很小，但参与FSH水平的调节。在原发性睾丸功能衰竭中，FSH水平升高与FSH脉冲幅度增加有关，并且在缺乏睾丸类固醇的情况下，可能还与FSH脉冲频率增加有关。
