Muto M, Kubo E, Kamisaku H, Sado T
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.
J Immunol. 1990 Feb 1;144(3):849-53.
Using an intrathymic injection assay on B10 Thy-1 congenic mice, it was demonstrated that thymic prelymphoma cells first developed within the thymuses from 4 to 8 days after split-dose irradiation and were detected in more than 63% of the test donor thymuses when examined at 21 and 31 days after irradiation. Moreover, some mice (25%) at 2 mo after split-dose irradiation had already developed thymic lymphomas in their thymuses. To characterize these thymic prelymphoma cells, the thymocytes from B10 Thy-1.1 mice 1 mo after irradiation were stained with anti-CD4 and anti-CD8 mAb and were sorted into four subpopulations. These fractionated cells were injected into the recipient thymuses to examine which subpopulation contained thymic prelymphoma cells. The results indicated that thymic prelymphoma cells existed mainly in CD4- CD8- and CD4- CD8+ thymocyte subpopulations and also in CD4+ CD8+ subpopulation. T cell lymphomas derived from CD4- CD8- prelymphoma cells had mainly CD4- CD8- or CD4- CD8+ phenotypes. T cell lymphomas developed from CD4- CD8+ prelymphoma cells mainly expressed CD4- CD8+ or CD4+ CD8+ phenotype. T cell lymphomas originating from CD4+ CD8+ prelymphoma cells were mainly CD4+ CD8+ but some CD4- CD8+ or CD4+ CD8- cells were also present. These thymic prelymphoma cells were further characterized phenotypically in relation to their expression of the marker defined by the mAb against J11d marker and TL-2 (thymus-leukemia) Ag, which is not expressed on normal thymocytes of B10.Thy-1.2 or B10.Thy-1.1 strain, but appears on the thymocytes of lymphomagenic irradiated mice. The results indicated that the prelymphoma cells existed in J11d+, TL-2+ cells.
通过对B10 Thy-1同源小鼠进行胸腺内注射试验，结果表明，分次剂量照射后4至8天，胸腺前淋巴瘤细胞首先在胸腺内形成，照射后21天和31天检查时，超过63%的供体胸腺中可检测到这些细胞。此外，分次剂量照射后2个月时，一些小鼠（25%）胸腺中已发生胸腺淋巴瘤。为了对这些胸腺前淋巴瘤细胞进行表征，对照射后1个月的B10 Thy-1.1小鼠的胸腺细胞用抗CD4和抗CD8单克隆抗体进行染色，并分选成四个亚群。将这些分选的细胞注射到受体胸腺中，以检查哪个亚群含有胸腺前淋巴瘤细胞。结果表明，胸腺前淋巴瘤细胞主要存在于CD4-CD8-和CD4-CD8+胸腺细胞亚群中，也存在于CD4+CD8+亚群中。源自CD4-CD8-前淋巴瘤细胞的T细胞淋巴瘤主要具有CD4-CD8-或CD4-CD8+表型。由CD4-CD8+前淋巴瘤细胞发展而来的T细胞淋巴瘤主要表达CD4-CD8+或CD4+CD8+表型。源自CD4+CD8+前淋巴瘤细胞的T细胞淋巴瘤主要为CD4+CD8+，但也存在一些CD4-CD8+或CD4+CD8-细胞。这些胸腺前淋巴瘤细胞进一步通过与抗J11d标志物和TL-2（胸腺白血病）抗原的单克隆抗体所定义的标志物表达相关的表型进行表征，该标志物在B10.Thy-1.2或B10.Thy-1.1品系的正常胸腺细胞上不表达，但在致淋巴瘤照射小鼠的胸腺细胞上出现。结果表明，前淋巴瘤细胞存在于J11d+、TL-2+细胞中。
