Department of General Surgery, No, 3 People's Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai 201900, China.
J Exp Clin Cancer Res. 2010 Nov 7;29(1):141. doi: 10.1186/1756-9966-29-141.
To study on expressions and clinical significances of CD133 protein and CD133 mRNA in primary lesion of gastric adenocarcinoma (GC).
Expressions of CD133 protein by immunostaining (99 cases) and CD133 mRNA by semi-quantitative RT-PCR (31 cases) were detected in primary lesion and in noncancerous gastric mucosa tissue (NCGT). Correlations of CD133 protein expression with clinicopathological parameters and post-operative survival were analyzed. Relations of CD133 mRNA level with Ki-67 labeling index (LI), and lymphatic metastasis were assessed too.
Brown particles indicating CD133 protein positivity occurred in some parts of tumor cells and epithelium. Expressive percentage of CD133 protein positivity was significantly higher in subgroups with >5 cm diameter (P = 0.041), later TNM stage (P = 0.044), severer lymph node metastasis (P = 0.017), occurrences of lymphatic invasion (P = 0.000) and vascular invasion (P = 0.000) respectively. Severer invasion depth (P = 0.011), lymph node metastasis occurrence (P = 0.043) and later TNM stage (P = 0.049) were the independent risk factors for CD133 protein expression. Average brightness scale value (BSV) of CD133 mRNA was significantly higher in subgroups with >5 cm diameter (P = 0.041), lymph node metastasis occurrence (P = 0.004) and in lower Ki-67 LI (P = 0.02). Relative analysis revealed that BSV of CD133 mRNA related positively to metastatic lymphatic nodes ratio (P = 0.008) and metastatic lymph node number (P = 0.009), but negatively to Ki-67 LI (P = 0.009). Survival of positive subgroup of CD 133 protein was significantly poorer (P = 0.047). Lymph node metastasis occurrence (P = 0.042), later TNM stage (P = 0.046) and CD 133 protein positive expression (P = 0.046) were respectively the independent risk factors to survival.
Higher expressive level of CD133 mRNA is associated to lower Ki-67 LI and severer lymphatic metastasis. Therefore, the expressive level of CD133 mRNA can play an appropriate role to reflect the status of lymph node metastasis and proliferation of GC. CD133 protein expression is closely related with larger tumor, later TNM stage, lymphtic metastasis and survival of GC.
研究 CD133 蛋白和 CD133mRNA 在胃腺癌（GC）原发灶中的表达及临床意义。
应用免疫组织化学法（99 例）和半定量 RT-PCR（31 例）检测 CD133 蛋白和 CD133mRNA 在原发灶和非癌性胃黏膜组织（NCGT）中的表达。分析 CD133 蛋白表达与临床病理参数及术后生存的关系。评估 CD133mRNA 水平与 Ki-67 标记指数（LI）和淋巴转移的关系。
肿瘤细胞和上皮部分出现 CD133 蛋白阳性的棕色颗粒。在直径>5cm（P=0.041）、较晚的 TNM 分期（P=0.044）、更严重的淋巴结转移（P=0.017）、淋巴管侵犯（P=0.000）和血管侵犯（P=0.000）亚组中，CD133 蛋白阳性率显著更高。更深的浸润深度（P=0.011）、淋巴结转移的发生（P=0.043）和较晚的 TNM 分期（P=0.049）是 CD133 蛋白表达的独立危险因素。CD133mRNA 的平均亮度标度值（BSV）在直径>5cm（P=0.041）、淋巴结转移发生（P=0.004）和较低的 Ki-67LI（P=0.02）亚组中显著较高。相关分析显示，CD133mRNA 的 BSV 与转移淋巴结比例（P=0.008）和转移淋巴结数（P=0.009）呈正相关，与 Ki-67LI（P=0.009）呈负相关。CD133 蛋白阳性亚组的生存明显较差（P=0.047）。淋巴结转移的发生（P=0.042）、较晚的 TNM 分期（P=0.046）和 CD133 蛋白阳性表达（P=0.046）是生存的独立危险因素。
较高的 CD133mRNA 表达水平与较低的 Ki-67LI 和更严重的淋巴转移相关。因此，CD133mRNA 的表达水平可以适当反映 GC 淋巴结转移和增殖的状态。CD133 蛋白的表达与较大的肿瘤、较晚的 TNM 分期、淋巴转移和 GC 的生存密切相关。
