Patel B, Mohammad R M, Blaustein J, al-Katib A
Department of Medicine, Wayne State University, Detroit, MI.
Am J Hematol. 1990 Mar;33(3):153-9. doi: 10.1002/ajh.2830330302.
A human common acute lymphoblastic leukemia (ALL) cell line, REH, was treated in vitro with gamma-interferon (gamma-IFN) or 12-O-tetradecanoylphorbol 13-acetate (TPA). Untreated (control) and treated cells were analyzed for changes in growth patterns, morphology, cytochemistry, surface phenotype, and terminal transferase (TdT) activity. TPA but not gamma-IFN induced further maturation of REH cells along the B-cell lineage. There was a dramatic decrease in CALLA expression, loss of TdT activity, induction of Leu M5, and increase in Leu 14 expression. TPA also induced monocytoid morphological features on REH cells. Enzymatically, the induced cells strongly expressed acid phosphatase (tartrate sensitive), alpha-naphthol acetate esterase (NAE), and periodic acid Schiff (PAS). We conclude that TPA induced monocytoid B-lymphocyte features on REH cells within the B-cell lineage, which should not be confused with monocytes/macrophage. The phenotype of cells in this stage is Leu 14+, Leu M5+, BL1+, Leu 12+, AcP+, PAS+, NAE+, CALLA-, TdT-, MO1-, and MO2-.
用人急性淋巴细胞白血病（ALL）细胞系REH在体外进行γ干扰素（γ-IFN）或十四酰佛波醇乙酯（TPA）处理。对未处理（对照）和处理后的细胞进行生长模式、形态学、细胞化学、表面表型和末端转移酶（TdT）活性变化的分析。TPA而非γ-IFN诱导REH细胞沿B细胞系进一步成熟。CALLA表达显著降低，TdT活性丧失，Leu M5诱导表达，Leu 14表达增加。TPA还诱导REH细胞出现单核细胞样形态特征。在酶学方面，诱导后的细胞强烈表达酸性磷酸酶（耐酒石酸）、α-萘酚乙酸酯酶（NAE）和过碘酸希夫（PAS）。我们得出结论，TPA在B细胞系内诱导REH细胞出现单核细胞样B淋巴细胞特征，不应与单核细胞/巨噬细胞混淆。此阶段细胞的表型为Leu 14+、Leu M5+、BL1+、Leu 12+、AcP+、PAS+、NAE+、CALLA-、TdT-、MO1-和MO2-。
