改良的具有增强固氮生物肥料潜力的环保型重组鱼腥藻 PCC7120 菌株。
Improved eco-friendly recombinant Anabaena sp. strain PCC7120 with enhanced nitrogen biofertilizer potential.
机构信息
Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
出版信息
Appl Environ Microbiol. 2011 Jan;77(2):395-9. doi: 10.1128/AEM.01714-10. Epub 2010 Nov 5.
Abstract
Photosynthetic, nitrogen-fixing Anabaena strains are native to tropical paddy fields and contribute to the carbon and nitrogen economy of such soils. Genetic engineering was employed to improve the nitrogen biofertilizer potential of Anabaena sp. strain PCC7120. Constitutive enhanced expression of an additional integrated copy of the hetR gene from a light-inducible promoter elevated HetR protein expression and enhanced functional heterocyst frequency in the recombinant strain. The recombinant strain displayed consistently higher nitrogenase activity than the wild-type strain and appeared to be in homeostasis with compatible modulation of photosynthesis and respiration. The enhanced combined nitrogen availability from the recombinant strain positively catered to the nitrogen demand of rice seedlings in short-term hydroponic experiments and supported better growth. The engineered strain is stable, eco-friendly, and useful for environmental application as nitrogen biofertilizer in paddy fields.
摘要
具有光合作用和固氮能力的鱼腥藻菌株是热带稻田的本地种，为土壤的碳氮循环做出贡献。本研究通过基因工程改良鱼腥藻 PCC7120 菌株的生物固氮能力。通过组成型表达一个来自光诱导启动子的额外整合的 hetR 基因拷贝，提高了重组菌株中 HetR 蛋白的表达水平和功能性异形胞的频率。与野生型菌株相比，重组菌株表现出更高的固氮酶活性，并且似乎通过光合作用和呼吸作用的兼容调节达到了稳态。在短期水培实验中，重组菌株增加的可利用氮含量满足了水稻幼苗的氮需求，并促进了更好的生长。该工程菌株稳定、环保，可作为生物固氮剂应用于稻田的环境中。
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