肠内营养对大鼠模型中小肠树突状细胞激活和浸润的影响。
Total parenteral-nutrition-mediated dendritic-cell activation and infiltration into the small intestine in a rat model.
机构信息
Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi, Yufu, Oita 879-5593, Japan.
出版信息
J Anesth. 2011 Feb;25(1):57-64. doi: 10.1007/s00540-010-1037-8. Epub 2010 Nov 6.
PURPOSE
Total parenteral nutrition (TPN) is commonly carried out in the clinical setting. However, effects of TPN on the immune system, including dendritic cells (DC), are not well understood. The purpose of this study was to determine whether TPN affects DC activation and infiltration into the intestinal barrier.
METHODS
Male Wistar rats were given conventional nutrition (CN) or TPN for 7 days. DCs were visualized by immunohistochemistry. Levels of nucleotide-binding oligomerization domain protein 2 (NOD2) and high-mobility group box 1 (HMGB1) protein were assessed by Western blot.
RESULTS
The number of DCs at the small intestinal barrier was significantly increased in the TPN group (9.2 ± 3.1 cells/microscopic field) compared with the CN group (0.5 ± 0.6 cells/microscopic field; p < 0.05), as were protein expression levels of NOD2 and HMGB1.
CONCLUSION
These results suggest that TPN increases activation and infiltration of DCs into the small intestine, potentially involving an increase in NOD2 and HMGB1 levels in the small intestine.
目的
全肠外营养（TPN）在临床中常被应用。然而，TPN 对免疫系统的影响，包括树突状细胞（DC），尚未完全明确。本研究旨在确定 TPN 是否会影响 DC 的活化和向肠道屏障的浸润。
方法
雄性 Wistar 大鼠接受常规营养（CN）或 TPN 治疗 7 天。通过免疫组织化学观察 DC。通过 Western blot 评估核苷酸结合寡聚结构域蛋白 2（NOD2）和高迁移率族蛋白 B1（HMGB1）蛋白的水平。
结果
与 CN 组（0.5±0.6 个/视野）相比，TPN 组的小肠屏障处 DC 数量明显增加（9.2±3.1 个/视野；p<0.05），NOD2 和 HMGB1 蛋白的表达水平也增加。
结论
这些结果表明，TPN 可增加 DC 向小肠的活化和浸润，可能涉及小肠中 NOD2 和 HMGB1 水平的增加。
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