Microbial Ecology Department, Institute of Botany, University of Warsaw, Warsaw, Poland.
FEMS Microbiol Ecol. 2011 Jan;75(1):89-98. doi: 10.1111/j.1574-6941.2010.00990.x. Epub 2010 Nov 9.
The community of picocyanobacteria inhabiting the Great Mazurian Lakes system (comprising lakes ranging from mesotrophic to hypertrophic) is dominated by phycoerythrin-rich cells, which outnumber phycocyanin-rich cells, even in hypertrophic lakes. The genetic diversity and phylogeny of 43 strains of picocyanobacteria isolated from four Mazurian lakes were studied by analyzing the nucleotide sequences of the 16S rRNA gene and cpcBA-IGS operon. Phylogenetic analyses assigned some of the strains to several previously described clusters (Groups A, B, C, E and I) and revealed the existence of a novel clade, Group M (Mazurian), which exhibited a low level of similarity to the other clusters. Both phycocyanin and phycoerythrin picocyanobacteria were assigned to this clade based on an analysis of the 16S rRNA gene. The cpcBA sequence analysis assigned only phycocyanin strains to Group M, whereas the phycoerythrin strains from the M ribogroup were assigned to Groups B and E. We hypothesize that Group M originally contained only phycocyanin picocyanobacteria. The phycoerythrin found in strains belonging to ribogroup M seems to have been acquired through horizontal gene transfer as an adaptation to the changing environment early in the ontogeny of these glacial lakes.
栖息于大马尔祖里亚湖系统（包括从中营养型到富营养型的湖泊）的小型蓝藻群落主要由富含藻红蛋白的细胞主导，甚至在富营养型湖泊中也是如此，这些细胞的数量超过了富含藻蓝蛋白的细胞。通过分析 16S rRNA 基因和 cpcBA-IGS 操纵子的核苷酸序列，研究了从四个马祖里湖分离出的 43 株小型蓝藻的遗传多样性和系统发育。系统发育分析将一些菌株分配到几个先前描述的聚类（A、B、C、E 和 I 组）中，并揭示了一个新的聚类，即 M 组（马祖里）的存在，该聚类与其他聚类的相似度较低。基于 16S rRNA 基因分析，将藻蓝蛋白和藻红蛋白小型蓝藻都分配到这个聚类中。cpcBA 序列分析仅将藻蓝蛋白菌株分配到 M 组，而来自 M 核糖体组的藻红蛋白菌株则分配到 B 组和 E 组。我们假设 M 组最初只包含藻蓝蛋白小型蓝藻。属于 M 核糖体组的菌株中发现的藻红蛋白似乎是通过水平基因转移获得的，作为对这些冰川湖早期发育过程中环境变化的适应。
