卤虫的延伸因子EF-1α被鸟嘌呤核苷酸类似物吡哆醛-5'-二磷酸-5'-鸟苷特异性标记在赖氨酸-63处。
Elongation factor EF-1 alpha from Artemia is specifically labeled at Lys-63 by the guanine nucleotide analog pyridoxal-5'-diphospho-5'-guanosine.
作者信息
Amons R, Schipper A, Möller W
机构信息
Department of Medical Biochemistry, Sylvius Laboratory, State University of Leiden, The Netherlands.
出版信息
Biochim Biophys Acta. 1990 Mar 1;1037(3):401-4. doi: 10.1016/0167-4838(90)90043-f.
PMID:2106916
Abstract
We have synthesized the guanine nucleotide analog pyridoxal-5'-diphospho-5'-guanosine. This compound specifically modifies a single lysine residue in elongation factor 1 alpha from Artemia, indicating that this residue is in close contact with the reactive part of the guanine nucleotide analog. This result is discussed in terms of the structure of the nucleotide-binding domain of the factor.
摘要
我们合成了鸟嘌呤核苷酸类似物吡哆醛-5'-二磷酸-5'-鸟苷。该化合物特异性修饰卤虫延伸因子1α中的单个赖氨酸残基，表明该残基与鸟嘌呤核苷酸类似物的反应部分紧密接触。根据该因子核苷酸结合结构域的结构对这一结果进行了讨论。
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