用荧光猝灭法研究钾一、二磷酸盐与牛血清白蛋白的相互作用。
Interaction of potassium mono and di phosphates with bovine serum albumin studied by fluorescence quenching method.
机构信息
Department of Physics, Annamalai University, Annamalainagr, 608 002 Tamilnadu, India.
出版信息
J Fluoresc. 2011 Mar;21(2):687-92. doi: 10.1007/s10895-010-0756-4. Epub 2010 Nov 12.
The interactions between potassium mono and di phosphates and bovine serum albumin (BSA) were studied using fluorescence spectroscopy (FS) and ultraviolet spectroscopy (UV). The experimental results showed that the potassium mono and di phosphates could insert into the BSA and quench the inner fluorescence of BSA by forming the potassium mono phosphate-BSA and pottassium di phosphate-BSA complexes. It was found that the static quenching was the main reason leading to the fluorescence quenching. It was conformed by XRD and SEM techniques.
采用荧光光谱法（FS）和紫外光谱法（UV）研究了磷酸一钾和磷酸二钾与牛血清白蛋白（BSA）的相互作用。实验结果表明，磷酸一钾和磷酸二钾可以插入 BSA 中，并通过形成磷酸一钾-BSA 和磷酸二钾-BSA 配合物来猝灭 BSA 的内荧光。研究发现，静态猝灭是导致荧光猝灭的主要原因。这一点通过 XRD 和 SEM 技术得到了证实。
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