College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, China.
Cell Biol Int. 2011 Aug;35(8):835-9. doi: 10.1042/CBI20100317.
We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGFP and differentiate in vitro. The results demonstrated that stem cells could be isolated from amniotic fluid of the EGFP gene transgenic porcine fetuses and could express EGFP and differentiate in vitro. Undifferentiated AFSs (amniotic fluid-derived stem cells) expressed POU5F1, THY1 and SOX2, while the following differentiation cells expressed markers for chondrogenic (COL2A1), osteogenic (osteocalcin and osteonectin) and neurogenic cells such as astrocyte (GFAP), oligodendrocyte (GALC) and neuron (NF, ENO2 and MAP).
我们之前获得了 EGFP（增强型绿色荧光蛋白）基因转基因猪胎儿。本研究的目的是：（i）确定是否可以从转基因猪胎儿的羊水中分离出干细胞；（ii）确定这些干细胞是否可以在体外表达 EGFP 并分化。结果表明，可从 EGFP 基因转基因猪胎儿的羊水中分离出干细胞，并可在体外表达 EGFP 并分化。未分化的 AFS（羊水来源的干细胞）表达 POU5F1、THY1 和 SOX2，而随后的分化细胞则表达软骨形成（COL2A1）、成骨（骨钙素和骨桥蛋白）和神经发生细胞的标志物，如星形胶质细胞（GFAP）、少突胶质细胞（GALC）和神经元（NF、ENO2 和 MAP）。
