多能性与细胞重编程：事实、假说、未解问题。

Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues.

机构信息

The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

出版信息

Cell. 2010 Nov 12;143(4):508-25. doi: 10.1016/j.cell.2010.10.008.

DOI:10.1016/j.cell.2010.10.008

PMID:21074044

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3032267/

Abstract

Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state. In addition, evidence has accumulated that distinct states of pluripotency can interconvert through the modulation of both cell-intrinsic and exogenous factors. To fully realize the potential of in vitro reprogrammed cells, we need to understand the molecular and epigenetic determinants that convert one cell type into another. Here we review recent advances in this rapidly moving field and emphasize unresolved and controversial questions.

摘要

通过异位表达特定转录因子将体细胞直接重编程为诱导多能干细胞，引发了关于分化细胞状态的表观遗传稳定性的基本问题。此外，有证据表明，通过调节细胞内和外源因素，不同的多能性状态可以相互转换。为了充分发挥体外重编程细胞的潜力，我们需要了解将一种细胞类型转化为另一种细胞类型的分子和表观遗传决定因素。在这里，我们回顾了该快速发展领域的最新进展，并强调了未解决和有争议的问题。