多能性与细胞重编程：事实、假说、未解问题。
Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues.
机构信息
The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
出版信息
Cell. 2010 Nov 12;143(4):508-25. doi: 10.1016/j.cell.2010.10.008.
Abstract
Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state. In addition, evidence has accumulated that distinct states of pluripotency can interconvert through the modulation of both cell-intrinsic and exogenous factors. To fully realize the potential of in vitro reprogrammed cells, we need to understand the molecular and epigenetic determinants that convert one cell type into another. Here we review recent advances in this rapidly moving field and emphasize unresolved and controversial questions.
摘要
通过异位表达特定转录因子将体细胞直接重编程为诱导多能干细胞，引发了关于分化细胞状态的表观遗传稳定性的基本问题。此外，有证据表明，通过调节细胞内和外源因素，不同的多能性状态可以相互转换。为了充分发挥体外重编程细胞的潜力，我们需要了解将一种细胞类型转化为另一种细胞类型的分子和表观遗传决定因素。在这里，我们回顾了该快速发展领域的最新进展，并强调了未解决和有争议的问题。
相似文献
1
Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues.多能性与细胞重编程：事实、假说、未解问题。
Cell. 2010 Nov 12;143(4):508-25. doi: 10.1016/j.cell.2010.10.008.
2
Concise review: Induced pluripotent stem cells versus embryonic stem cells: close enough or yet too far apart?简明综述：诱导多能干细胞与胚胎干细胞：足够接近还是相差甚远？
Stem Cells. 2012 Jan;30(1):33-41. doi: 10.1002/stem.700.
3
Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.Trib2 调节胚胎干细胞的多能性并提高重编程效率。
Exp Mol Med. 2017 Nov 24;49(11):e401. doi: 10.1038/emm.2017.191.
4
Transcriptional analysis of pluripotency reveals the Hippo pathway as a barrier to reprogramming.多能性的转录分析揭示 Hippo 通路是重编程的障碍。
Hum Mol Genet. 2012 May 1;21(9):2054-67. doi: 10.1093/hmg/dds023. Epub 2012 Jan 27.
5
High throughput sequencing identifies an imprinted gene, Grb10, associated with the pluripotency state in nuclear transfer embryonic stem cells.高通量测序鉴定出一个与核移植胚胎干细胞多能性状态相关的印记基因Grb10。
Oncotarget. 2017 Jul 18;8(29):47344-47355. doi: 10.18632/oncotarget.17185.
6
Reprogramming somatic cells towards pluripotency by cellular fusion.通过细胞融合将体细胞重编程为多能性。
Curr Opin Genet Dev. 2012 Oct;22(5):459-65. doi: 10.1016/j.gde.2012.07.005. Epub 2012 Aug 3.
7
Direct Reprogramming of Human Amniotic Fluid Stem Cells by OCT4 and Application in Repairing of Cerebral Ischemia Damage.OCT4对人羊水干细胞的直接重编程及其在修复脑缺血损伤中的应用
Int J Biol Sci. 2016 Mar 11;12(5):558-68. doi: 10.7150/ijbs.11051. eCollection 2016.
8
Conversion of partially reprogrammed cells to fully pluripotent stem cells is associated with further activation of stem cell maintenance- and gamete generation-related genes.部分重编程细胞向完全多能干细胞的转化与干细胞维持和配子生成相关基因的进一步激活有关。
Stem Cells Dev. 2014 Nov 1;23(21):2637-48. doi: 10.1089/scd.2014.0020. Epub 2014 Aug 20.
9
Ground rules of the pluripotency gene regulatory network.多能性基因调控网络的基本规则。
Nat Rev Genet. 2017 Mar;18(3):180-191. doi: 10.1038/nrg.2016.156. Epub 2017 Jan 3.
10
The role of the reprogramming method and pluripotency state in gamete differentiation from patient-specific human pluripotent stem cells.重编程方法和多能性状态在患者特异性人多能干细胞向配子分化中的作用。
Mol Hum Reprod. 2018 Apr 1;24(4):173-184. doi: 10.1093/molehr/gay007.
引用本文的文献
1
Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during development.整合因子促进TFIID与RNA聚合酶II的结合，以在发育过程中维持多能性。
Mol Cell. 2025 Aug 7;85(15):2937-2955.e10. doi: 10.1016/j.molcel.2025.07.006.
2
Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.利用绒毛膜绒毛活检获取患者特异性滋养层干细胞。
Commun Biol. 2025 Jul 1;8(1):964. doi: 10.1038/s42003-025-08393-1.
3
The interplay of cellular senescence and reprogramming shapes the biological landscape of aging and cancer revealing novel therapeutic avenues.细胞衰老与重编程之间的相互作用塑造了衰老和癌症的生物学格局，揭示了新的治疗途径。
Front Cell Dev Biol. 2025 Apr 28;13:1593096. doi: 10.3389/fcell.2025.1593096. eCollection 2025.
4
CORN 2.0 - Condition Orientated Regulatory Networks 2.0.CORN 2.0 - 面向疾病状态的调控网络2.0版
Comput Struct Biotechnol J. 2025 Apr 3;27:1518-1528. doi: 10.1016/j.csbj.2025.04.003. eCollection 2025.
5
Autophagy, ER-phagy and ER Dynamics During Cell Differentiation.细胞分化过程中的自噬、内质网自噬与内质网动态变化
J Mol Biol. 2025 Sep 15;437(18):169151. doi: 10.1016/j.jmb.2025.169151. Epub 2025 Apr 11.
6
Deciphering normal and cancer stem cell niches by spatial transcriptomics: opportunities and challenges.通过空间转录组学解析正常和癌症干细胞微环境：机遇与挑战
Genes Dev. 2025 Jan 7;39(1-2):64-85. doi: 10.1101/gad.351956.124.
7
Reprogramming stem cells in regenerative medicine.再生医学中的干细胞重编程。
Smart Med. 2022 Dec 25;1(1):e20220005. doi: 10.1002/SMMD.20220005. eCollection 2022 Dec.
8
Discoveries from human stem cell research in space that are relevant to advancing cellular therapies on Earth.来自太空人类干细胞研究的发现，这些发现与推进地球上的细胞疗法相关。
NPJ Microgravity. 2024 Aug 21;10(1):88. doi: 10.1038/s41526-024-00425-0.
9
Generation of induced pluripotent stem cells from the Asian bats.从亚洲蝙蝠中诱导产生多能干细胞。
Int J Vet Sci Med. 2024 Aug 12;12(1):81-90. doi: 10.1080/23144599.2024.2384835. eCollection 2024.
10
Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.利用绒毛膜绒毛活检来获取患者特异性滋养层干细胞。
medRxiv. 2024 Jul 23:2022.12.07.22283218. doi: 10.1101/2022.12.07.22283218.
本文引用的文献
1
Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors.通过定义因子将成纤维细胞直接重编程为功能性心肌细胞。
Cell. 2010 Aug 6;142(3):375-86. doi: 10.1016/j.cell.2010.07.002.
2
Lab-specific gene expression signatures in pluripotent stem cells.多能干细胞中的特定于实验室的基因表达特征。
Cell Stem Cell. 2010 Aug 6;7(2):258-62. doi: 10.1016/j.stem.2010.06.016.
3
Chromatin structure and gene expression programs of human embryonic and induced pluripotent stem cells.人类胚胎干细胞和诱导多能干细胞的染色质结构和基因表达程序。
Cell Stem Cell. 2010 Aug 6;7(2):249-57. doi: 10.1016/j.stem.2010.06.015.
4
Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells.细胞起源类型影响小鼠诱导多能干细胞的分子和功能特性。
Nat Biotechnol. 2010 Aug;28(8):848-55. doi: 10.1038/nbt.1667. Epub 2010 Jul 19.
5
Epigenetic memory in induced pluripotent stem cells.诱导多能干细胞中的表观遗传记忆。
Nature. 2010 Sep 16;467(7313):285-90. doi: 10.1038/nature09342.
6
Functional genomics reveals a BMP-driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming.功能基因组学揭示了在体细胞重编程起始过程中 BMP 驱动的间质到上皮的转变。
Cell Stem Cell. 2010 Jul 2;7(1):64-77. doi: 10.1016/j.stem.2010.04.015. Epub 2010 Jun 17.
7
Reprogramming of human peripheral blood cells to induced pluripotent stem cells.人外周血细胞重编程为诱导多能干细胞。
Cell Stem Cell. 2010 Jul 2;7(1):20-4. doi: 10.1016/j.stem.2010.06.002.
8
Reprogramming of T cells from human peripheral blood.重编程人外周血中的T细胞。
Cell Stem Cell. 2010 Jul 2;7(1):15-9. doi: 10.1016/j.stem.2010.06.004.
9
Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells.从人类终末分化的循环T细胞中诱导生成多能干细胞。
Cell Stem Cell. 2010 Jul 2;7(1):11-4. doi: 10.1016/j.stem.2010.06.003.
10
Genome-wide reprogramming in the mouse germ line entails the base excision repair pathway.在小鼠生殖系中进行全基因组重编程需要碱基切除修复途径。
Science. 2010 Jul 2;329(5987):78-82. doi: 10.1126/science.1187945.
Zotero 插件