Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, 29 13th Street, TEDA, Tianjin 300457, People's Republic of China.
Can J Microbiol. 2010 Nov;56(11):925-33. doi: 10.1139/w10-075.
Seventeen virulent bacteriophages specific to Pseudomonas aeruginosa strains were isolated by screening various environmental samples. These isolated bacteriophages were grouped based on results obtained from restriction fragment analysis of phage genomes, random amplification of polymorphic DNA (RAPD) typing, morphology observations under transmission electron microscope, and host range analysis. All 17 bacteriophages are double-stranded DNA viruses and can be divided into 5 groups based on DNA restriction profiles. A set of 10-mer primers was used in RAPD typing of phages, and similar conclusions were obtained as for restriction fragment analysis. One phage was randomly selected from each of the 5 groups for morphology observations. Four of them had an icosahedral head with a long contractile tail, belonging to the Myoviridae family, and one phage had an icosahedral head with a short tail, thereby belonging to the Podoviridae family. Host range experiments were conducted on 7 laboratory strains and 12 clinical strains of P. aeruginosa. The results showed that 13 phages had the same infection profile, killing 8 out of 19 tested P. aeruginosa strains, and the remaining 4 phages had different and unique infection profiles. This study highlights the diversity of bacteriophages specific to P. aeruginosa in the environment.
从各种环境样本中筛选出了 17 株特异性针对铜绿假单胞菌菌株的烈性噬菌体。根据噬菌体基因组的限制性片段分析、随机扩增多态性 DNA (RAPD) 分型、透射电镜下形态观察和宿主范围分析的结果，对这些分离的噬菌体进行了分组。所有 17 株噬菌体均为双链 DNA 病毒，根据 DNA 限制图谱可分为 5 组。使用一组 10 聚体引物对噬菌体进行 RAPD 分型，得到的结论与限制性片段分析相似。从每组中随机选择一个噬菌体进行形态观察。其中 4 个噬菌体头部呈二十面体，带有长的收缩尾，属于肌尾噬菌体科，另一个噬菌体头部呈二十面体，带有短尾，属于短尾噬菌体科。对 7 株实验室菌株和 12 株临床分离的铜绿假单胞菌进行了宿主范围实验。结果表明，有 13 株噬菌体具有相同的感染谱，杀死了 19 株测试铜绿假单胞菌菌株中的 8 株，其余 4 株噬菌体具有不同且独特的感染谱。本研究强调了环境中特异性针对铜绿假单胞菌的噬菌体的多样性。
