Department of Radiology, Seoul National University Hospital, Seoul, Korea.
Korean J Radiol. 2010 Nov-Dec;11(6):673-82. doi: 10.3348/kjr.2010.11.6.673. Epub 2010 Oct 29.
To evaluate transplanted porcine pancreatic islets in the kidney capsules of diabetic mice using a clinically approved superparamagnetic iron oxide (SPIO) and a 1.5T MR scanner.
Various numbers of porcine pancreatic islets labeled with Resovist, a carboxydextran-coated SPIO, were transplanted into the kidney capsules of normal mice and imaged with a 3D FIESTA sequence using a 1.5T clinical MR scanner. Labeled (n = 3) and unlabeled (n = 2) islets were transplanted into the kidney capsules of streptozotocin-induced diabetic mice. Blood glucose levels and MR signal intensities were monitored for 30 days post-transplantation.
There were no significant differences in viability or insulin secretion between labeled and unlabeled islets. A strong correlation (r(2) > 0.94) was evident between the number of transplanted islets and T(2) relaxation times quantified by MRI. Transplantation with labeled or unlabeled islets helped restore normal sustained glucose levels in diabetic mice, and nephrectomies induced the recurrence of diabetes. The MR signal intensity of labeled pancreatic islets decreased by 80% over 30 days.
The transplantation of SPIO-labeled porcine islets into the kidney capsule of diabetic mice allows to restore normal glucose levels, and these islets can be visualized and quantified using a 1.5T clinical MR scanner.
使用临床批准的超顺磁氧化铁（SPIO）和 1.5T MR 扫描仪评估糖尿病小鼠肾囊内移植的猪胰岛。
用羧基葡聚糖包裹的 SPIO（Resovist）标记不同数量的猪胰岛，将其移植到正常小鼠的肾囊中，并使用 1.5T 临床 MR 扫描仪的 3D FIESTA 序列进行成像。将标记（n = 3）和未标记（n = 2）的胰岛移植到链脲佐菌素诱导的糖尿病小鼠的肾囊中。在移植后 30 天监测血糖水平和 MR 信号强度。
标记和未标记胰岛的活力或胰岛素分泌没有显著差异。通过 MRI 定量的 T2 弛豫时间与移植胰岛数量之间存在很强的相关性（r²＞0.94）。标记或未标记胰岛的移植有助于恢复糖尿病小鼠的正常持续血糖水平，并且肾切除术会导致糖尿病复发。标记的胰岛的 MR 信号强度在 30 天内降低了 80%。
将 SPIO 标记的猪胰岛移植到糖尿病小鼠的肾囊中可以恢复正常的血糖水平，并且可以使用 1.5T 临床 MR 扫描仪对这些胰岛进行可视化和定量。
