Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark.
Cell Biol Int. 2011 Jun;35(6):579-86. doi: 10.1042/CBI20100441.
Mechanical forces are crucial in the regulation of cell morphology and function. At the cellular level, these forces influence myoblast differentiation and fusion. In this study, we applied mechanical stimuli to embryonic muscle cells using magnetic microbeads, a method shown to apply stress to specific receptors on the cell surface. We showed that mechanical stimuli promote an increase in FAK (focal adhesion kinase) phosphorylation. In order to further shed light in the process of myoblast-induced differentiation by mechanical stimuli, we performed a proteomic analysis. Thirteen proteins were found to be affected by mechanical stimulation including galectin-1, annexin III and RhoGDI (Rho guanine-nucleotide-dissociation inhibitor). In this study, we demonstrate how the combination of this method of mechanical stimuli and proteomic analysis can be a powerful tool to detect proteins that are potentially interacting in biochemical pathways or complex cellular mechanisms during the process of myoblast differentiation. We determined an increase in expression and changes in cellular localization of galectin-1 in mechanically stimulated myoblasts. A potential involvement of galectin-1 in myoblast differentiation is presented.
机械力在调节细胞形态和功能中起着至关重要的作用。在细胞水平上，这些力影响成肌细胞的分化和融合。在本研究中，我们使用磁性微珠向胚胎肌肉细胞施加机械刺激，这种方法已被证明可以向细胞表面的特定受体施加应力。我们表明，机械刺激促进粘着斑激酶（FAK）磷酸化的增加。为了进一步阐明机械刺激引起的成肌细胞诱导分化的过程，我们进行了蛋白质组学分析。发现有 13 种蛋白质受到机械刺激的影响，包括半乳糖凝集素-1、膜联蛋白 III 和 Rho GDP 解离抑制剂（RhoGDI）。在这项研究中，我们展示了如何将这种机械刺激方法与蛋白质组学分析相结合，成为一种强大的工具，用于检测在成肌细胞分化过程中，可能在生化途径或复杂细胞机制中相互作用的蛋白质。我们确定了在机械刺激的成肌细胞中半乳糖凝集素-1的表达增加和细胞定位的变化。提出了半乳糖凝集素-1可能参与成肌细胞分化。
