锌指核糖核酸酶结构域蛋白 1（ZNRD1）在白血病细胞血管生成中的作用。

Role of ZNRD1 (zinc ribbon domain-containing 1) in angiogenesis of leukaemia cells.

机构信息

State Key Laboratory of Tumor Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xian 710032, Shaanxi Province, Peoples Republic of China.

出版信息

Cell Biol Int. 2011 Apr;35(4):321-4. doi: 10.1042/CBI20100506.

DOI:10.1042/CBI20100506

PMID:21080911

Abstract

Here, we have first investigated the roles of ZNRD1 in angiogenesis of leukaemia. The leukaemia cell line K562 was transfected with the vector that included the full-length cDNA of ZNRD1, then the growth and angiogenesis of cells were detected. Up-regulation of ZNRD1 could significantly inhibit the growth of cells, reduce tumour microvessel densities and inhibit the VEGF (vascular endothelial growth factor) production. The results of human miRNA array and real-time PCR showed that ZNRD1 could significantly up-regulate the expression of miR-214 and down-regulate the expression of miR-296. Taken together, ZNRD1 might inhibit tumour angiogenesis and could be considered as a target for leukaemia therapy.

摘要

在这里，我们首次研究了 ZNRD1 在白血病血管生成中的作用。将包含 ZNRD1 全长 cDNA 的载体转染白血病细胞系 K562 后，检测细胞的生长和血管生成。ZNDR1 的上调可显著抑制细胞生长，降低肿瘤微血管密度，并抑制 VEGF（血管内皮生长因子）的产生。人 miRNA 芯片和实时 PCR 的结果表明，ZNDR1 可显著上调 miR-214 的表达，下调 miR-296 的表达。综上所述，ZNDR1 可能抑制肿瘤血管生成，可作为白血病治疗的靶点。