通过纳米粒子上的界面 PCR 进行快速 DNA 检测。
Rapid DNA detection by interface PCR on nanoparticles.
机构信息
School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, PR China.
出版信息
Biosens Bioelectron. 2011 Jan 15;26(5):2495-9. doi: 10.1016/j.bios.2010.10.043. Epub 2010 Oct 31.
A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined based on quenched fluorescence intensity with 136 bp as the lower limit of effective range. And the real sample detections were also achieved successfully by this developed method. Therefore, this DNA detection method has the potential to be the powerful gene diagnostic tool.
一种新型的快速 DNA 检测方法，基于通过聚合酶链反应（PCR）使金纳米粒子（GNPs）荧光猝灭的量子点（QDs），得到了开发。在概念验证实验中，基于被猝灭的荧光强度，可以确定从 152 到 1003 个碱基对（bp）长度的扩增子 DNA，136 bp 为有效范围的下限。并且该方法还成功地实现了对实际样品的检测。因此，这种 DNA 检测方法具有成为强大基因诊断工具的潜力。
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