National Instrumentation Center for Environmental Management, Seoul National University, Seoul 151-921, Korea.
Microsc Microanal. 2011 Feb;17(1):118-24. doi: 10.1017/S1431927610093967. Epub 2010 Nov 19.
High-resolution imaging and quantitative surface analysis of epicuticular waxes and epistomatal chambers of pine species were performed by field emission scanning electron microscopy and white light scanning interferometry. Both juvenile and adult needles were collected from the two-year-old seedlings of Pinus rigida and Pinus densiflora and subjected to surface observations. Epicuticular wax structures developed on the cuticle layer as well as in the epistomatal chambers and appeared to occlude the cavities in the two pine species. The stomata of P. densiflora were characterized by more distinctly raised rings around openings than P. rigida. The most common epicuticular wax structures of the two pine species included tubules with terminal openings and coiled rodlets. Wax platelets were deposited on epistomatal chambers. Either rodlets or tubules seemed to be longer and thicker in P. rigida than those in P. densiflora. White light scanning interferometry revealed quantitative surface profiles, demonstrating more ridged (ca. 4 μm high) stomatal apertures and nearly twofold deeper (ca. 20 μm deep) epistomatal chambers of P. densiflora than those of P. rigida. These results suggest that white light scanning interferometry can be applied to unravel the quantitative surface features of epicuticular sculptures on plant leaves.
通过场发射扫描电子显微镜和白光扫描干涉仪对松属植物的表皮蜡质和表皮腔的高分辨率成像和定量表面分析。从 2 年生的赤松和油松幼苗中采集幼叶和成熟针叶进行表面观察。表皮蜡质结构在角质层以及表皮腔内发育，似乎堵塞了这两个松属物种的腔室。油松的气孔比赤松的开口周围的凸起环更为明显。这两个松属物种最常见的表皮蜡质结构包括带有末端开口的管状结构和螺旋状的杆状小体。蜡质小板沉积在表皮腔内。在赤松中，杆状小体或管状结构似乎比在油松中更长更厚。白光扫描干涉仪揭示了定量的表面轮廓，表明油松的气孔开口更具脊状（约 4 μm 高），而表皮腔深度几乎是赤松的两倍（约 20 μm 深）。这些结果表明，白光扫描干涉仪可用于揭示植物叶片表皮纹饰的定量表面特征。
