Faculty of Life Sciences and Dental School, Manchester Academic Health Sciences Centre, Michael Smith Building, University of Manchester, Manchester, United Kingdom.
Dev Dyn. 2010 Dec;239(12):3481-91. doi: 10.1002/dvdy.22483.
Mutation of SATB2 causes cleft palate in humans. To understand the role of SATB2 function in palatogenesis, SATB2 analyses in vertebrate model systems will be essential. To facilitate these analyses, we have performed a cross-species comparison of SATB2 structure and function across three vertebrate model systems: mouse, chick, and zebrafish. We find that the SATB2 transcript is highly conserved across human, mouse, chick, and zebrafish, especially within the Satb2 functional domains. Furthermore, our expression analyses demonstrate that SATB2 is likely to have similar functions in vertebrate model organisms and humans during development of the facial processes and secondary palate. Together, these data suggest an evolutionary conserved role for SATB2 during development of the face and palate across vertebrates. Moreover, expression of zebrafish satb2 in the anterior neurocranium supports the utility of the anterior neurocranium as a simplified model of amniote palatogenesis.
SATB2 突变导致人类腭裂。为了了解 SATB2 功能在腭形成中的作用，在脊椎动物模型系统中对 SATB2 进行分析将是至关重要的。为了促进这些分析，我们在三个脊椎动物模型系统：小鼠、鸡和斑马鱼中进行了 SATB2 结构和功能的跨物种比较。我们发现 SATB2 转录本在人类、小鼠、鸡和斑马鱼中高度保守，尤其是在 Satb2 功能结构域内。此外，我们的表达分析表明，SATB2 在面部过程和次级腭的发育过程中，可能在脊椎动物模型生物和人类中具有相似的功能。这些数据共同表明，SATB2 在脊椎动物面部和腭的发育过程中具有进化保守的作用。此外，斑马鱼 satb2 在颅前神经嵴中的表达支持了颅前神经嵴作为羊膜动物腭形成简化模型的实用性。
