Sun Ya-jing, Yu Shi-zhu, Sun Cui-yun, Wang Qian, Jin Shu-mei, Wu Wei-xiang, An Tong-ling
Department of Neuropathology, Neurology Institute, Tianjin Medical University General Hospital, China.
Zhonghua Bing Li Xue Za Zhi. 2010 Sep;39(9):606-10.
To investigate the relationship between chromosomal genomic DNA imbalance in medulloblastoma (MB), and the age and gender.
The gains and losses of chromosomal genomic DNA in 16 MBs were analyzed using comparative genomic hybridization.
The gains and(or) losses were found in 15 of the 16 cases. There was not significant difference (P > 0.05) between the total gains (10/16) and losses (11/16). Both of their differences had also no significance between different age and gender groups (P > 0.05). In 15 cases with gains and(or) losses, single-, two-, three- and multi-chromosome genomic DNA imbalances were 3/15, 4/15, 1/15 and 7/15 respectively. Eleven gain zones (+5q, +6q, +7q, +11q, +15q, +17p, +17q, +19q, +20q, +21q, +Xp) and twenty-five loss zones (-1p, -1q, -2p, -2q, -3q, -4p, -6p, -6q, -8p, -8q, -10p, -10q, -11p, -14q, -16p, -16q, -17p, -18p, -18q, -19p, -19q, -20p, -20q, -Xp, -Xq) were detected in those tumors. +7q (6/16), +17q (6/16), -14q (5/16) and -10q (3/16) were the most frequent, but -14q only occurred in the cases of > 10-year-old.
Most MBs have chromosomal genomic DNA imbalances. The frequent imbalance zones are mainly at the long arms of some chromosomes. +7q, +17q, -14q and -10q correlate closely to development of the tumors. -14q is important factor to result in MBs of > 10-year-old group. MB has possibly different molecular genetics subtype.
探讨髓母细胞瘤(MB)染色体基因组DNA失衡与年龄、性别的关系。
采用比较基因组杂交技术分析16例MB的染色体基因组DNA的增减情况。
16例中有15例存在染色体基因组DNA的增加和(或)减少。总增加(10/16)与总减少(11/16)之间差异无统计学意义(P>0.05)。不同年龄组和性别组之间其差异也无统计学意义(P>0.05)。在15例有增加和(或)减少的病例中,单条、两条、三条和多条染色体基因组DNA失衡分别为3/15、4/15、1/15和7/15。在这些肿瘤中检测到11个增加区(+5q、+6q、+7q、+11q、+15q、+17p、+17q、+19q、+20q、+21q、+Xp)和25个减少区(-1p、-1q、-2p、-2q、-3q、-4p、-6p、-6q、-8p、-8q、-10p、-10q、-11p、-14q、-16p、-16q、-17p、-18p、-18q、-19p、-19q、-20p、-20q、-Xp、-Xq)。+7q(6/16)、+17q(6/16)、-14q(5/16)和-10q(3/16)最为常见,但-14q仅出现在年龄>10岁的病例中。
多数MB存在染色体基因组DNA失衡。常见的失衡区主要位于某些染色体的长臂。+7q、+17q、-14q和-10q与肿瘤发生密切相关。-14q是导致10岁以上组MB的重要因素。MB可能存在不同的分子遗传学亚型。