Centro de Estudos em Estresse Oxidativo (Lab. 32), PPG Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Brain Res. 2011 Jan 19;1369:60-73. doi: 10.1016/j.brainres.2010.11.042. Epub 2010 Nov 17.
Vitamin A is important for both development and maintenance of adult brain homeostasis. However, excessive vitamin A exposure has been linked to cognitive impairments and may induce congenital defects, including neuronal malformations. Recently, we demonstrated that vitamin A supplementation is able to alter behavioral parameters and induce a pro-oxidant state in hippocampus and striatum of adult male rat. Thus, the aim of the present work was to investigate the effects of vitamin A supplementation in pregnant and nursing rats on maternal and offspring striatum and hippocampus. Wistar female rats (7 per group) were orally supplemented with retinyl palmitate (2500, 12,500 and 25,000 IU/kg/day) or saline (control) throughout pregnancy and nursing. Homing test was performed at postnatal days (PND) 5 and 10 for offspring, while open field test (OFT) was carried out at PND19 and 20 for dams and offspring, respectively. Redox parameters were evaluated at PND21 for both. Vitamin A supplementation during pregnancy and nursing increased superoxide dismutase/catalase (SOD/CAT) ratio and oxidative damage in maternal and offspring striatum and hippocampus. Additionally, supplementation induced behavioral alterations. In conclusion, we suggest some caution regarding vitamin A intake during pregnancy and breastfeeding, since oxidative stress can disturb several biological phenomena, including neuronal signaling and neurotransmission, which may induce several behavioral deficits.
维生素 A 对成人大脑的发育和维持稳态都很重要。然而，过量的维生素 A 暴露与认知障碍有关，并且可能导致先天缺陷，包括神经元畸形。最近，我们证明了维生素 A 补充剂能够改变成年雄性大鼠海马体和纹状体的行为参数，并诱导其产生促氧化状态。因此，本研究旨在探讨在怀孕和哺乳期大鼠中补充维生素 A 对母体和后代纹状体和海马体的影响。Wistar 雌性大鼠（每组 7 只）在整个孕期和哺乳期经口给予棕榈酸视黄酯（2500、12500 和 25000 IU/kg/天）或生理盐水（对照）。对后代在出生后第 5 天和第 10 天进行归巢测试，而对母鼠和后代分别在出生后第 19 天和第 20 天进行旷场测试。在第 21 天对两者的氧化还原参数进行评估。孕期和哺乳期补充维生素 A 增加了母体和后代纹状体和海马体中超氧化物歧化酶/过氧化氢酶（SOD/CAT）的比值和氧化损伤。此外，补充剂还诱导了行为改变。总之，我们建议在怀孕期间和母乳喂养期间要谨慎摄入维生素 A，因为氧化应激会干扰包括神经元信号传递和神经递质传递在内的多种生物学现象，从而可能导致多种行为缺陷。
