通过活细胞中单分子追踪观察到的kv2.1通道的异常扩散。

Anomalous diffusion of kv2.1 channels observed by single molecule tracking in live cells.

作者信息

Weigel Aubrey V, Tamkun Michael M, Krapf Diego

机构信息

School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:3005-8. doi: 10.1109/IEMBS.2010.5626150.

DOI:10.1109/IEMBS.2010.5626150

PMID:21095721

Abstract

Kv2.1 are voltage gated potassium channels that form long-lived clusters on the surface of mammalian cells. We have used single molecule tracking to study the interesting dynamics of these channels in live HEK cells. Both the channels inside the clusters and non-clustering channels are found to follow anomalous subdiffusion. The effect of actin cytoskeleton on the diffusion properties of the channels is also investigated in the presence of cytochalasin D, a F-actin binding drug that blocks actin polymerization.

摘要

Kv2.1是电压门控钾通道，在哺乳动物细胞表面形成长期存在的簇。我们使用单分子追踪技术研究了这些通道在活的人胚肾（HEK）细胞中的有趣动力学。发现簇内的通道和非簇状通道均遵循反常亚扩散。还在细胞松弛素D（一种阻断肌动蛋白聚合的F-肌动蛋白结合药物）存在的情况下，研究了肌动蛋白细胞骨架对通道扩散特性的影响。