一种用于在深层组织中定位光源的无光学参数方法。

An optical-parameters-free method for locating light source in deep tissue.

作者信息

Quan Qinghua, Zheng Zhen, Yao Yong, Gu Jia, Wang Lei

机构信息

IBHE, Shenzhen Institute of Technology, Chinese Academy of Sciences, Shenzhen, China.

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:3033-6. doi: 10.1109/IEMBS.2010.5626145.

DOI:10.1109/IEMBS.2010.5626145

PMID:21095728

Abstract

Bioluminescence of luciferase-expressing cells in live animals is a promising technique for image guided tumor resection. However, requiring exact optical tissue parameters is an underlying problem of diffusion equation when recovering bioluminescent images of luciferase activity within a volume using emission optical signal data from internal light sources. In this paper, an equivalence method based on the equation of radiative transfer (ERT) is proposed to locate bioluminescence source without optical tissue parameters. The solution of equivalence method is satisfied with the unique of localization as well as the complex tissue environment. The reconstructed images of bioluminescence are presented with simulated heterogeneous tissue models, and an 8.7% relative error of the source localization is obtained.

摘要

在活体动物中，表达荧光素酶的细胞的生物发光是一种用于图像引导肿瘤切除的很有前景的技术。然而，当使用来自内部光源的发射光信号数据恢复体积内荧光素酶活性的生物发光图像时，需要精确的光学组织参数是扩散方程的一个潜在问题。本文提出了一种基于辐射传输方程（ERT）的等效方法，用于在无需光学组织参数的情况下定位生物发光源。等效方法的解满足定位的唯一性以及复杂的组织环境。利用模拟的异质组织模型给出了生物发光的重建图像，并且获得了源定位8.7%的相对误差。