Yang Yun-gao, Liu Ya-wei, Hua He-yu, Li Li-jie
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Nov;30(11):2443-5.
To investigate effect of Sanhuangyinchi decoction (SHYCD) on liver damage and the pro-apoptotic factor caspase-3 in rats with acute hepatic failure.
SD rats were randomly divided into blank control group, model group, Angongniuhuang group (AGNH) and SHYCD group. Acute hepatic failure was induced in the rats by intraperitoneal injections of D-GaLN and LPS, and the death rate, ALT, TBIL, PT and caspase-3 activity was observed or tested.
At 36 h after the injections, the death rate of the rats was 74.29% (26/35) in the model group, 31.43% (11/35) in AGNH group and 28.57%(10/35) in SHYCD group. The death rate, ALT, TBIL, PT and caspase-3 activity in AGNH and SHYCD groups were significantly lower than those in the model group (P<0.01). SHYCD showed stronger effect than AGNH in depressing TBIL and the activity of caspase-3 (P<0.05).
SHYCD can improve the liver function and inhibit the activity of caspase-3 in rats, which can be the possible mechanism for its therapeutic effect against acute hepatic failure.
探讨三黄茵陈汤对急性肝衰竭大鼠肝损伤及促凋亡因子半胱天冬酶-3的影响。
将SD大鼠随机分为空白对照组、模型组、安宫牛黄组和三黄茵陈汤组。通过腹腔注射D-氨基半乳糖和脂多糖诱导大鼠急性肝衰竭，观察或检测大鼠的死亡率、谷丙转氨酶（ALT）、总胆红素（TBIL）、凝血酶原时间（PT）及半胱天冬酶-3活性。
注射后36小时，模型组大鼠死亡率为74.29%（26/35），安宫牛黄组为31.43%（11/35），三黄茵陈汤组为28.57%（10/35）。安宫牛黄组和三黄茵陈汤组的死亡率、ALT、TBIL、PT及半胱天冬酶-3活性均显著低于模型组（P<0.01）。三黄茵陈汤在降低TBIL及半胱天冬酶-3活性方面的作用强于安宫牛黄组（P<0.05）。
三黄茵陈汤可改善大鼠肝功能并抑制半胱天冬酶-3活性，这可能是其治疗急性肝衰竭的作用机制。
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