Kato Koichi, Iwata Hiroo
Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Methods Mol Biol. 2011;706:181-90. doi: 10.1007/978-1-61737-970-3_15.
Genome-wide functional annotation of genes is one of the major challenges in current biology. Such investigation requires a high-throughput methodology for efficient and parallel overexpression or silencing of multiplexed genes in living cells. The transfection method described here employs an electric pulse and a cell-chip technology, and provides the possibility of analyzing gene functions in a high-throughput manner.
基因的全基因组功能注释是当前生物学面临的主要挑战之一。此类研究需要一种高通量方法，以便在活细胞中对多个基因进行高效、并行的过表达或沉默。本文所述的转染方法采用电脉冲和细胞芯片技术，并提供了高通量分析基因功能的可能性。
