Department of Chemical and Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul, 136-701, Republic of Korea.
Curr Microbiol. 2011 Mar;62(3):1023-7. doi: 10.1007/s00284-010-9818-8. Epub 2010 Nov 21.
A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml(-1) of lipids in A. chrysogenum.
建立了一种利用油红 O 快速测定阿江榄仁内生真菌细胞内脂含量的方法。改良的油红 O 可以在 75°C 下处理 10 分钟后强烈染色阿江榄仁内生真菌的脂类。研究结果表明，本文建立的油红 O 染色方法可用于定量检测阿江榄仁内生真菌中 0.1-5mg/ml 的脂类。
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