AQP1 和 eNOS 在绝经后大鼠膀胱中的表达。
The Expression of AQP1 and eNOS in Menopausal Rat Urinary Bladder.
机构信息
Department of Urology, Chonnam National University Medical School, Gwangju, Korea.
出版信息
Int Neurourol J. 2010 Aug;14(2):78-85. doi: 10.5213/inj.2010.14.2.78. Epub 2010 Aug 31.
PURPOSE
Aquaporins (AQPs) have been reported to be expressed in rat and human urothelium. Nitric oxide (NO) is thought to play an important role in the bladder overactivity related to menopause. The purpose of this study was to investigate the effect of hormonal alteration on the expression of AQP1 and eNOS in menopausal rat urinary bladder.
MATERIALS AND METHODS
Female Sprague-Dawley rats (230-240 g, N=30) were divided into three groups: control (N=10), bilateral ovariectomy (Ovx, N=10), and bilateral ovariectomy followed by subcutaneous injections of 17β-estradiol (50 mg/kg/day, Ovx+Est, N=10). After 4 weeks, urodynamic studies measuring the contraction interval and contraction pressure were done. The expression and cellular localization of AQP1 and eNOS were determined by performing Western blotting and immunohistochemistry on the rat urinary bladder.
RESULTS
The approximate contraction interval (min) was significantly decreased in the Ovx group (3.9±0.25) compared to the control group (6.7±0.15), and was increased after estrogen treatment (9.7±0.22) (p<0.05). The AQP1 and eNOS immunoreactivities were localized in the same areas: capillaries, arterioles, and venules of the lamina propria. The protein expression of AQP1 was not changed significantly, whereas eNOS expression was significantly decreased in the Ovx group and restored to the control value in the Ovx+Est group.
CONCLUSIONS
This study showed that ovariectomy causes a significant change in e-NOS expression without a change in AQP1 in menopausal rat urinary bladder. This may imply that e-NOS has a functional role in the bladder overactivity that occurs in association with menopause.
目的
水通道蛋白（AQP）已被报道在大鼠和人尿路上皮中表达。一氧化氮（NO）被认为在与绝经相关的膀胱过度活动中发挥重要作用。本研究旨在探讨激素变化对绝经大鼠膀胱中 AQP1 和 eNOS 表达的影响。
材料和方法
雌性 Sprague-Dawley 大鼠（230-240 g，N=30）分为三组：对照组（N=10）、双侧卵巢切除术（Ovx，N=10）和双侧卵巢切除术后皮下注射 17β-雌二醇（50 mg/kg/天，Ovx+Est，N=10）。4 周后，进行尿动力学研究，测量收缩间隔和收缩压。通过对大鼠膀胱进行 Western blot 和免疫组织化学分析，测定 AQP1 和 eNOS 的表达和细胞定位。
结果
Ovx 组的近似收缩间隔（min）明显缩短（3.9±0.25），与对照组（6.7±0.15）相比，而雌激素治疗后增加（9.7±0.22）（p<0.05）。AQP1 和 eNOS 免疫反应性定位于同一区域：固有层的毛细血管、小动脉和小静脉。AQP1 蛋白表达无明显变化，而 eNOS 表达在 Ovx 组显著降低，在 Ovx+Est 组恢复至对照组水平。
结论
本研究表明，卵巢切除术后绝经大鼠膀胱中 e-NOS 表达显著改变，而 AQP1 无变化。这可能意味着 e-NOS 在与绝经相关的膀胱过度活动中具有功能作用。
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