[人结直肠癌细胞系SW480裸鼠移植瘤中增殖诱导配体（APRIL）对肿瘤生长及凋亡的影响]

[Effect of APRIL on growth and apoptosis in transplanted tumor with human colorectal cancer cell line SW480 in nude mice].

作者信息

Wang Jing-chun, Ding Wei-feng, Sun Bao-lan, Jing Rong-rong, Huang Hua, Wang Hui-min

机构信息

Center of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2010 Aug;32(8):570-4.

PMID:21122406

Abstract

OBJECTIVE

To study the effect of pGCsi-H1-APRIL on the growth of human colorectal cancer cells in transplated tumor in nude mice and to improve the effect of APRIL on proliferation and apoptosis of colorectal cancer (CRC).

METHODS

Human CRC model was established in nude mice, and the nude mice were treated with APRIL siRNA twice per week for 2 weeks. APRIL mRNA expression was surveyed by PCR and APRIL protein expression was detected by immunohistochemistry. The expression of PCNA protein was detected by ELISA. The expression of bcl-2 and bcl-xl was assessed by immunohistochemical staining, and TUNEL staining was used to detect apoptosis.

RESULTS

The expression of APRIL mRNA in the APRIL siRNA group was (0.13 ± 0.05) × 10(-3), significantly lower than that in the vector group (0.95 ± 0.04) × 10(-3) and the PBS group (0.96 ± 0.05) × 10(-3). The expression of APRIL protein in the APRIL siRNA group was (87.5 ± 5.0)% lower than that in the vector and PBS groups (P < 0.05). APRIL siRNA significantly suppressed the growth of SW480 tumor: the IR (inhibitory rate) of APRIL siRNA group was (60.7 ± 1.5)% (P < 0.05). The expression of PCNA in APRIL siRNA group was (176.8 ± 18.1) ng/ml, was (56.5 ± 2.0)% lower than that of PBS group (328.4 ± 22.8) ng/ml. Furthermore, the expressions of anti-apoptosis proteins bcl-2 and bcl-xl of APRIL siRNA group were (82.6 ± 4.5)% and (79.2 ± 3.5)% lower than those of the PBS group. The apoptotic rate of the APRIL siRNA group was 40.1% ± 2.5%, significantly higher than that in the vector group (2.5 ± 0.1)% and PBS group (2.5 ± 0.2)% (P < 0.05).

CONCLUSION

APRIL siRNA may significantly suppress the growth and promote apoptosis in transplanted tumor of human colorectal cancer in nude mice. APRIL may become a candidate gene of gene therapy of human colorectal cancer.

摘要

目的

研究pGCsi-H1-APRIL对裸鼠移植瘤中人大肠癌细胞生长的影响，以提高APRIL对大肠癌（CRC）增殖和凋亡的作用。

方法

在裸鼠中建立人CRC模型，每周对裸鼠进行2次APRIL siRNA处理，共2周。通过PCR检测APRIL mRNA表达，通过免疫组化检测APRIL蛋白表达。通过ELISA检测PCNA蛋白表达。通过免疫组化染色评估bcl-2和bcl-xl的表达，并使用TUNEL染色检测细胞凋亡。

结果

APRIL siRNA组中APRIL mRNA的表达为(0.13±0.05)×10(-3)，显著低于载体组(0.95±0.04)×10(-3)和PBS组(0.96±0.05)×10(-3)。APRIL siRNA组中APRIL蛋白的表达比载体组和PBS组低(87.5±5.0)%(P<0.05)。APRIL siRNA显著抑制SW480肿瘤的生长：APRIL siRNA组的IR（抑制率）为(60.7±1.5)%(P<0.05)。APRIL siRNA组中PCNA的表达为(176.8±18.1)ng/ml，比PBS组(328.4±22.8)ng/ml低(56.5±2.0)%。此外，APRIL siRNA组抗凋亡蛋白bcl-2和bcl-xl的表达比PBS组低(82.6±4.5)%和(79.2±3.5)%。APRIL siRNA组的凋亡率为40.1%±2.5%，显著高于载体组(2.5±0.1)%和PBS组(2.5±0.2)%(P<0.05)。