Department of Chemical Pathology, Beaumont Hospital, Dublin 9, Ireland.
Ann Clin Biochem. 2011 Jan;48(Pt 1):41-4. doi: 10.1258/acb.2010.010062. Epub 2010 Dec 1.
Normetanephrine and metanephrine are intermediate metabolites of noradrenaline and adrenaline metabolism. To assess whether normetanephrine and metanephrine analysis may aid in the diagnosis of Neuroblastoma, a reference interval for these metabolites must first be established.
The overall aim of this study was to establish a paediatric age-related reference interval for the measurement of total fractionated metanephrines.
A total of 267 urine samples were analysed following acid hydrolysis. This releases the metanephrines from their sulphate-bound metabolites. The samples were analysed using reverse phase high-performance liquid chromatography with electro-chemical detection on a Gilson automated sequential trace enrichment of dialysate sample system.
Data were analysed using Minitab Release version 14. Outliers were removed using the Dixon/Reed one-third rule. Partitioning of the age groups was achieved using Harris and Boyd's standard normal deviate test. Non-parametric analysis of the data was performed, followed by the establishment of the 2.5th and the 97.5th reference limits.
The established reference intervals are described in Table 2.
去甲变肾上腺素和变肾上腺素是去甲肾上腺素和肾上腺素代谢的中间产物。为了评估去甲变肾上腺素和变肾上腺素分析是否有助于神经母细胞瘤的诊断，必须首先建立这些代谢物的参考区间。
本研究的总体目的是建立一个与儿童年龄相关的总分离变肾上腺素测量参考区间。
对 267 份尿液样本进行酸水解分析。这会将变肾上腺素从其硫酸盐结合的代谢物中释放出来。使用吉尔森自动顺序痕量透析液样品系统上的反相高效液相色谱和电化学检测来分析样本。
使用 Minitab Release 版本 14 分析数据。使用 Dixon/Reed 三分之一规则去除离群值。使用 Harris 和 Boyd 的标准正态偏差测试对年龄组进行分区。对数据进行非参数分析，然后建立第 2.5 和第 97.5 个参考限。
表 2 中描述了已建立的参考区间。
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