结合激光捕获显微切割和蛋白质组学：方法学和临床应用。

Combining laser capture microdissection and proteomics: methodologies and clinical applications.

机构信息

Department of Neurological Surgery, Vanderbilt University School of Medicine, Nashville, TN, USA.

出版信息

Proteomics Clin Appl. 2010 Feb;4(2):116-23. doi: 10.1002/prca.200900138. Epub 2009 Nov 19.

DOI:10.1002/prca.200900138

Abstract

Laser capture microdissection (LCM) has become an important tool for biomedical research. Various molecular biology techniques have been combined with LCM to reveal molecular profiles at an unprecedented tissue resolution. Proteomics is among those techniques that have proved fruitful in combination with LCM. This review provides an overview of the various proteomic techniques that have been developed for analyzing cells obtained using LCM. Methods for analyzing microdissected cells for various proteomic techniques are described and compared. In addition, various applications based on LCM and proteomics are discussed.

摘要

激光捕获显微切割（LCM）已成为生物医学研究的重要工具。各种分子生物学技术已与 LCM 结合使用，以以前所未有的组织分辨率揭示分子谱。蛋白质组学是与 LCM 结合使用已被证明卓有成效的技术之一。本文综述了用于分析使用 LCM 获得的细胞的各种蛋白质组学技术。描述并比较了用于分析各种蛋白质组学技术的微切割细胞的方法。此外，还讨论了基于 LCM 和蛋白质组学的各种应用。