De Marinis L, Mancini A, D'Amico C, Calabró F, Zuppi P, Fiumara C, Barini A
Institut of Endocrinology, Catholic University School of Medicine, Rome, Italy.
Neuroendocrinology. 1990 Jun;51(6):717-20. doi: 10.1159/000125416.
It has been previously shown that in normal women during the periovulatory period, prolactin (PRL) levels increase after the administration of nonspecific stimuli, such as growth hormone-releasing hormone or gonadotropin-releasing hormone (GnRH). In order to gain insight into the mechanism of this response, we have tested the effect of a naloxone infusion (1.6 mg/h) on the PRL response to GnRH in 5 normal females, aged 20-27 years, tested during the periovulatory period. Naloxone was administered starting 60 min before GnRH administration (100 micrograms as an i.v. bolus). Naloxone clearly blunted the PRL response (basal 10.7 +/- 1.7 ng/ml, peak 11.8 +/- 0.2 ng/ml at 30 min, versus: basal 9.0 +/- 0.5 ng/ml, peak 20.6 +/- 3.9 ng/ml at 45 min after GnRH alone; significance of difference between peaks: p less than 0.05). A secondary late increase of PRL levels was observed, reaching about 60% of basal levels at 120 min (16.8 +/- 2.6 ng/ml). These data indicate that periovulatory PRL dynamics are altered by naloxone administration and suggest a possible involvement of opioid peptides in the 'paradoxical' PRL response to GnRH in normal subjects.
先前的研究表明，在正常女性的排卵期，给予生长激素释放激素或促性腺激素释放激素（GnRH）等非特异性刺激后，催乳素（PRL）水平会升高。为了深入了解这种反应的机制，我们测试了纳洛酮输注（1.6毫克/小时）对5名年龄在20 - 27岁的正常女性在排卵期对GnRH的PRL反应的影响。在给予GnRH（100微克静脉推注）前60分钟开始给予纳洛酮。纳洛酮明显减弱了PRL反应（基础值10.7±1.7纳克/毫升，30分钟时峰值11.8±0.2纳克/毫升，相比之下：单独给予GnRH后45分钟时基础值9.0±0.5纳克/毫升，峰值20.6±3.9纳克/毫升；峰值之间差异的显著性：p＜0.05）。观察到PRL水平有二次晚期升高，在120分钟时达到基础水平的约60%（16.8±2.6纳克/毫升）。这些数据表明，给予纳洛酮会改变排卵期PRL的动态变化，并提示阿片肽可能参与正常受试者对GnRH的“反常”PRL反应。
