Zhang Jing, Zheng Yue-Hui, Zheng Li-Ping
The Assistance Reproduction Branch, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Feb;24(1):86-9.
To study the expression and control of telomerase in rat preantral ovary.
Added different factors to the preantral ovarian granulosa cells, then using TRAP-ELISA to analyze the expression of telomerase.
Telomerase activation was detected in granulosa cells. Telomerase activation could be significantly enhanced by hcG, FSH, verapamil and dbcAMP, and it was significantly reduced by antisense-c-myb ODN treatment. We also used radioimmunoassay to determine proterone and estrogen in these cell culture mediums. It was found that these two hormones' secretion were raised under verapamil and FSH; no change under hcG and dbcAMllted with telomerase activity. In MTT assay, the antisense hTERT ODN could significantly inhibited the proliferation of ovarian granulosa cells.
It is suggested that telomerase activation is present in ovary antral granulosa cell. Anti-c-myb might inhibit telomerase activation, while FSH, hcG, verapamil, dbcAMP might enhance the telomerase activation.
研究端粒酶在大鼠窦前卵泡卵巢中的表达及调控。
向窦前卵泡卵巢颗粒细胞中添加不同因子，然后用端粒重复序列扩增-酶联免疫吸附测定法分析端粒酶的表达。
在颗粒细胞中检测到端粒酶激活。人绒毛膜促性腺激素、促卵泡激素、维拉帕米和双丁酰环磷腺苷可显著增强端粒酶激活，而反义c-myb寡脱氧核苷酸处理可显著降低端粒酶激活。我们还用放射免疫分析法测定了这些细胞培养基中的孕酮和雌激素。发现这两种激素在维拉帕米和促卵泡激素作用下分泌增加；在人绒毛膜促性腺激素和双丁酰环磷腺苷作用下无变化，且与端粒酶活性相关。在噻唑蓝比色法中，反义人端粒酶逆转录酶寡脱氧核苷酸可显著抑制卵巢颗粒细胞的增殖。
提示窦前卵泡卵巢颗粒细胞存在端粒酶激活。抗c-myb可能抑制端粒酶激活，而促卵泡激素、人绒毛膜促性腺激素、维拉帕米、双丁酰环磷腺苷可能增强端粒酶激活。
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