Gorriti Arilmi, Arroyo Jorge, Quispe Fredy, Cisneros Braulio, Condorhuamán Martín, Almora Yuan, Chumpitaz Víctor
Laboratorio de Farmacognosia y Medicina Tradicional, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2010 Sep;27(3):352-60. doi: 10.1590/s1726-46342010000300007.
To evaluate the oral toxicity at 60 days and to determine the lethal dose 50 (LD 50) of raw sacha inchi (Plukenetia volubilis L.) and linseed (Linum ussitatisimum) oils in Holtzman rats and mice of the strain Balb C57 respectively.
For the evaluation of the oral toxicity of repeated doses for 60 days, 24 male Holtzman rats were used, divided in three groups of 8 each, the groups were: physiologic saline solution 4 mL/kg (FSS), sacha inchi oil 0.5 mL/kg (SI05) and linseed oil 0.5 mL/kg (L05), during the experiment the body weight was controlled weekly, and signs of toxicity in the research groups, as well as total cholesterol, HDL, glucose, triglycerides and alkaline phosphatase at days 30 and 60 after initiating the experiment. For the evaluation of the LD50 male mice of the Balb C57 strain were used in groups of 10 animals, and they were administered increasing oral doses of raw oils until reaching 1 mL/kg (37 g/kg).
The serum parameters in the rats indicated there is no toxicity at 60 days and that the administration of the oils lowered the levels of cholesterol, triglycerides and increased the HDL in comparison with the control group. The LD50 shows that the raw sacha inchi and linseed oils have doses above 37 g/kg of body weight.
Sacha inchi and linseed oils are harmless at 60 days and present a LD50 above the 37 g/kg of animal.
分别评估霍兹曼大鼠和Balb C57品系小鼠摄入生印加果（Plukenetia volubilis L.）油和亚麻籽油60天的经口毒性，并确定半数致死剂量（LD50）。
为评估60天重复经口给药的毒性，选用24只雄性霍兹曼大鼠，分为3组，每组8只，分组如下：4 mL/kg生理盐水溶液（FSS）、0.5 mL/kg印加果油（SI05）和0.5 mL/kg亚麻籽油（L05）。实验期间每周监测体重，并观察各研究组的毒性体征，以及在实验开始后第30天和第60天检测总胆固醇、高密度脂蛋白、葡萄糖、甘油三酯和碱性磷酸酶。为评估LD50，选用Balb C57品系雄性小鼠，每组10只，给予递增剂量的生油经口灌胃，直至达到1 mL/kg（37 g/kg）。
大鼠血清参数表明，60天时无毒性，与对照组相比，给予油类可降低胆固醇和甘油三酯水平，并提高高密度脂蛋白水平。LD50表明，生印加果油和亚麻籽油的半数致死剂量高于37 g/kg体重。
印加果油和亚麻籽油在60天时无危害，其LD50高于37 g/kg动物体重。
