用于在光学显微镜下改善形态学、形态计量学和免疫组织化学研究的甲基丙烯酸乙二醇酯包埋：以睾丸为模型

Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model.

作者信息

Chiarini-Garcia Hélio, Parreira Gleydes Gambogi, Almeida Fernanda R C L

机构信息

Laboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

出版信息

Methods Mol Biol. 2011;689:3-18. doi: 10.1007/978-1-60761-950-5_1.

DOI:10.1007/978-1-60761-950-5_1

PMID:21153783

Abstract

Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.

摘要

甲基丙烯酸乙二醇酯（GMA）是一种可与水和乙醇混溶的塑料树脂，是一种便于用于光学显微镜包埋的介质，比石蜡包埋具有许多优势。GMA主要由于能准确评估细胞学细节，从而改善了组织学、形态计量学和免疫组织化学评估。本章重点介绍我们在GMA处理方面的经验，并详细描述我们用于睾丸评估的固定、包埋和染色方法。