[0.7兆帕（7个大气压）气压下大鼠脊髓突触体甘氨酸摄取的变化]
[Changes of glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa (7ATA) air pressure].
作者信息
Bi Gang, Zhang Jian, Yang Tao, Liu Jing-Chang
机构信息
Diving Medical Research Department, Navy Medical Research Institute, Shanghai 200433, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Aug;23(3):334-7.
AIM
To study changes of function of transmitter glycine in nitrogen narcosis.
METHODS
Synaptosomes of rat spinal cord were prepared. Glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa (7ATA) hyperbaric air pressure was observed by the methods of isotope.
RESULTS
Glycine uptake slowed down and took a longer period of time to reach saturation in 0.7 MPa (7ATA). The maximum glycine uptake was lessened. Vm was diminished, but Km was increased. Vm rose in 0.7 MPa (7ATA) when corticosterone was added.
CONCLUSION
When nitrogen narcosis arose in 0.7 MPa (7ATA), the function of transporters of glycine re-uptake was reduced, the affinity of glycine for transporters subsided. Corticosterone was conductive to the recovery of the function of glycine transporters of high affinity.
目的
研究氮麻醉时递质甘氨酸功能的变化。
方法
制备大鼠脊髓突触体。采用同位素方法观察0.7MPa（7ATA）高压空气压力下大鼠脊髓突触体对甘氨酸的摄取情况。
结果
在0.7MPa（7ATA）时，甘氨酸摄取减慢，达到饱和所需时间延长。最大甘氨酸摄取量减少。Vm降低，但Km增加。添加皮质酮后，0.7MPa（7ATA）时Vm升高。
结论
0.7MPa（7ATA）出现氮麻醉时，甘氨酸重摄取转运体功能降低，甘氨酸对转运体的亲和力下降。皮质酮有助于高亲和力甘氨酸转运体功能的恢复。
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