Nishino E, Matsuzaki N, Masuhiro K, Kameda T, Taniguchi T, Takagi T, Saji F, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
J Clin Endocrinol Metab. 1990 Aug;71(2):436-41. doi: 10.1210/jcem-71-2-436.
We examined the capacity of trophoblast-derived interleukin-6 (IL-6) to stimulate secretion of placental hormones, including hCG. IL-6 stimulated hCG secretion by trophoblasts to a level similar to that stimulated by a GnRH analog. The analog, however, released hCG by an IL-6-independent mechanism because PM-1, a monoclonal antibody specific for IL-6 receptors (R), failed to block GnRH-mediated responses, but completely blocked IL-6-mediated hCG secretion, suggesting the existence of two distinct regulatory pathways for hCG release. Immunohistochemical analysis with another IL-6-R-specific antibody, MT-18, showed that IL-6-R was located only on the trophoblast layer of the placenta. Our data revealed the existence of a local regulatory network by which trophoblast-derived IL-6 interacts with IL-6-R on the trophoblasts, resulting in hCG release. Thus, two different regulatory networks, an IL-6 and IL-6-R system and a GnRH and GnRH-R system, regulate hCG release by human trophoblasts independently.
我们研究了滋养层来源的白细胞介素-6（IL-6）刺激包括人绒毛膜促性腺激素（hCG）在内的胎盘激素分泌的能力。IL-6刺激滋养层细胞分泌hCG的水平与促性腺激素释放激素（GnRH）类似物刺激的水平相似。然而，该类似物通过一种不依赖IL-6的机制释放hCG，因为一种对IL-6受体（R）具有特异性的单克隆抗体PM-1未能阻断GnRH介导的反应，但完全阻断了IL-6介导的hCG分泌，这表明存在两种不同的hCG释放调节途径。用另一种IL-6-R特异性抗体MT-18进行的免疫组织化学分析表明，IL-6-R仅位于胎盘的滋养层。我们的数据揭示了一个局部调节网络的存在，通过该网络，滋养层来源的IL-6与滋养层细胞上的IL-6-R相互作用，从而导致hCG释放。因此，两个不同的调节网络，即IL-6和IL-6-R系统以及GnRH和GnRH-R系统，独立调节人滋养层细胞释放hCG。
