Type 2 Diabetes Pathophysiology Group, Steno Diabetes Center, Gentofte, Denmark.
Obesity (Silver Spring). 2011 Jun;19(6):1304-6. doi: 10.1038/oby.2010.311. Epub 2010 Dec 16.
Retinol-binding protein 4 (RBP4) is a plasma protein which is elevated in obesity and type 2 diabetes. We aimed to investigate whether RBP4 represents a mechanism underlying the associations between low birth weight (LBW), high-fat diet, and insulin resistance. Forty-six young, lean men with low (n = 20) or normal (n = 26) birth weight underwent a 5-day high-fat high-calorie (HFHC) dietary intervention. In vivo glucose metabolism was assessed by euglycemic-hyperinsulinemic clamp, glucose tracer and intravenous glucose tolerance test techniques. Body composition was measured by a dual-energy x-ray absorptiometry scan, and plasma RBP4 by an enzyme-linked immunosorbent assay. RBP4 was not associated with birth weight, but with BMI (β = 0.9 µg/ml (0.08; 1.8) (95% confidence interval), P = 0.03) and plasma levels of low-density lipoprotein cholesterol (β = 5.3 µg/ml (1.9; 8.7), P = 0.03) and triglycerides (β = 15.4 µg/ml (9.5; 21.3), P < 0.001). Under baseline diet conditions, RBP4 was associated with decreased disposition index (D(i)) (β = -2.4% (-4.5%; -0.2%), P = 0.04) and increased basal hepatic glucose production rate (HGP) (β = 0.02 mg kg(-1) min(-1) (0.002; 0.04), P = 0.03), but not associated with peripheral glucose disposal rate or hepatic insulin resistance index. RBP4 levels were not influenced by overfeeding or related to peripheral and hepatic insulin resistance provoked by the dietary intervention. In conclusion, plasma RBP4 in young men associates with components of the metabolic syndrome, but is not determined by birth weight and seems not to be involved in short-term high-fat diet-induced insulin resistance.
视黄醇结合蛋白 4（RBP4）是一种血浆蛋白，在肥胖症和 2 型糖尿病患者中升高。我们旨在研究 RBP4 是否代表了低出生体重（LBW）、高脂肪饮食和胰岛素抵抗之间关联的机制。46 名年轻、瘦的男性，出生体重低（n = 20）或正常（n = 26），进行了为期 5 天的高脂肪高热量（HFHC）饮食干预。通过正葡萄糖高胰岛素钳夹、葡萄糖示踪和静脉葡萄糖耐量试验技术评估体内葡萄糖代谢。通过双能 X 射线吸收仪扫描测量身体成分，通过酶联免疫吸附试验测量血浆 RBP4。RBP4 与出生体重无关，但与 BMI（β = 0.9 µg/ml（0.08；1.8）（95%置信区间），P = 0.03）和血浆中低密度脂蛋白胆固醇（β = 5.3 µg/ml（1.9；8.7），P = 0.03）和甘油三酯（β = 15.4 µg/ml（9.5；21.3），P < 0.001）水平相关。在基础饮食条件下，RBP4 与降低的处置指数（D(i)）（β = -2.4%（-4.5%；-0.2%），P = 0.04）和增加的基础肝葡萄糖生成率（HGP）（β = 0.02 mg kg(-1) min(-1)（0.002；0.04），P = 0.03）相关，但与外周葡萄糖处置率或肝胰岛素抵抗指数无关。RBP4 水平不受过度喂养影响，也与饮食干预引起的外周和肝胰岛素抵抗无关。总之，年轻男性的血浆 RBP4 与代谢综合征的成分相关，但不受出生体重的影响，似乎与短期高脂肪饮食引起的胰岛素抵抗无关。
