Department of Pharmacology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China.
Int J Oncol. 2011 Feb;38(2):465-71. doi: 10.3892/ijo.2010.871. Epub 2010 Dec 14.
Endostatin is an anti-angiogenic agent that blocks endothelial cell proliferation, tumor growth, and metastasis. Several lines of direct evidence show that gliomas express high levels of endostatin. However, the anti-angiogenic activity and cellular mechanisms of endostatin from tumor cells have not been completely elucidated. In this study, we established C6 glioblastoma (GBM) xenografts in nude mice by subcutaneously injecting glioma cell lines, C6-null cells, and stable transfected-C6 cells overexpressing mock vector (C6-mock) and endostatin (C6-endo). We found that overexpression of endostatin in C6-endo cells significantly suppressed the expression of VEGF in tumor cells in vivo as well as in vitro. Furthermore, the tumor growth derived from C6-endo cells was inhibited. Our data demonstrate that endostatin could play a direct role in inhibiting tumor cells, and suggest that enhancing endostatin expression in gliomas could be an effective treatment strategy.
内皮抑素是一种抗血管生成剂，可阻止内皮细胞增殖、肿瘤生长和转移。有几条直接证据表明，神经胶质瘤表达高水平的内皮抑素。然而，肿瘤细胞的内皮抑素的抗血管生成活性和细胞机制尚未完全阐明。在这项研究中，我们通过皮下注射神经胶质瘤细胞系 C6 空载体细胞、稳定转染的mock 载体（C6-mock）和内皮抑素（C6-endo）的 C6 细胞，在裸鼠中建立了 C6 神经胶质瘤（GBM）异种移植物。我们发现，C6-endo 细胞中内皮抑素的过表达显著抑制了体内和体外肿瘤细胞中 VEGF 的表达。此外，C6-endo 细胞来源的肿瘤生长受到抑制。我们的数据表明内皮抑素可以直接抑制肿瘤细胞，并提示增强神经胶质瘤中的内皮抑素表达可能是一种有效的治疗策略。
