加蓬患者的急性登革病毒 2 感染与早期先天免疫反应有关，包括强烈的干扰素α产生。

BACKGROUND

Dengue is now a leading cause of morbidity and mortality throughout the tropics. We conducted the first ex vivo study of dengue fever (DF) in African patients infected during the first Gabonese dengue virus 2 (DENV-2) outbreak in 2007, in order to investigate cytokine production, including the antiviral cytokine IFN-α, reported to be a potent inhibitor of DENV replication in vitro.

METHODS

Levels of 50 cytokines, chemokines and growth factors were measured in plasma from 36 patients with DENV-2 infection, and in uninfected controls, using Luminex multiplex technology. The results were interpreted according to the day of sampling after symptom onset. PBMC from six patients were also studied for T lymphocyte cell surface marker expression by flow cytometry.

RESULTS

Acute DENV-2 infection elicited high levels of several pro-inflammatory cytokines (IL-6 and IL-17), chemokines (MIF, RANTES, IP-10 and MCP-1) and growth factors (G-CSF, GM-CSF and VEGF-A). We also observed high levels of IFN-α for the first time in adult DF patients, and CD4+ and CD8+ T cell activation at symptom onset.

CONCLUSION

Acute DENV-2 infection in African patients elicits a strong innate response involving IFN-α production, as well as an adaptive immune response.

背景

登革热现已成为热带地区发病率和死亡率的主要原因。我们首次对 2007 年在加蓬发生的首例登革热病毒 2 型（DENV-2）暴发期间感染的非洲患者的登革热（DF）进行了离体研究，以调查细胞因子的产生情况，包括抗病毒细胞因子 IFN-α，据报道，它是体外抑制 DENV 复制的有效抑制剂。

方法

使用 Luminex 多重分析技术，对 36 例 DENV-2 感染患者和未感染对照者的血浆中的 50 种细胞因子、趋化因子和生长因子的水平进行了测量。根据发病后第几天进行采样来解释结果。还通过流式细胞术研究了六位患者的 PBMC 对 T 淋巴细胞表面标志物的表达。

结果

急性 DENV-2 感染可引起几种促炎细胞因子（IL-6 和 IL-17）、趋化因子（MIF、RANTES、IP-10 和 MCP-1）和生长因子（G-CSF、GM-CSF 和 VEGF-A）的高水平表达。我们还首次在成年 DF 患者中观察到高水平的 IFN-α，并在发病时观察到 CD4+和 CD8+T 细胞的激活。