Coombs G H, Wolf C R, Morrison V M, Craft J A
Department of Zoology, University of Glasgow, U.K.
Mol Biochem Parasitol. 1990 Jun;41(1):17-24. doi: 10.1016/0166-6851(90)90092-z.
Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b5 and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b5 reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.
用杜氏利什曼原虫感染小鼠会导致几种参与外源性物质代谢的肝脏酶活性降低。感染肝脏的微粒体膜中细胞色素P450、b5以及NADPH - 细胞色素P450还原酶的含量减少。几种细胞色素P450同工酶（P450 - PB1、P450 - PB3、P450 - PCN和P450 - UT1）以及P450介导的反应（氨基比林脱甲基酶、苯胺羟化酶、苄非他明脱甲基酶和乙氧香豆素脱乙基酶）受到的影响类似。肝脏微粒体膜制剂对两种致癌物（亚硝基二甲胺和7,12 - 二甲基苯并[a]蒽）的代谢也减少。利什曼原虫感染导致胞质环氧化物水解酶增加，微粒体环氧化物水解酶和NADH - 细胞色素b5还原酶未受影响。结果表明，与未感染动物相比，感染利什曼原虫的动物对外源毒素的反应可能会发生改变。
