State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China.
Acta Biochim Biophys Sin (Shanghai). 2011 Jan;43(1):38-44. doi: 10.1093/abbs/gmq112.
Poliovirus genomic RNA replication, protein translation, and virion assembly are performed in the cytoplasm of host cells. However, this does not mean that there is no relationship between poliovirus infection and the cellular nucleus. In this study, recombinant fluorescence-tagged poliovirus 3CD and 3C' proteins were shown to be expressed mainly in the cytoplasm of Vero cells in the absence of other viral proteins. However, upon poliovirus infection, many of these proteins redistributed to the nucleus, as well as to the cytoplasm. A series of transfection experiments revealed that the poliovirus 2A(pro) was responsible for the same redistribution of 3CD and 3C' proteins to the nucleus. Furthermore, a mutant 2A(pro) protein lacking protease activity abrogated this effect. The poliovirus 2A(pro) protein was also found to co-localize with the Nup153 protein, a component of the nuclear pore complexes on the nuclear envelope. These data provide further evidence that there are intrinsic interactions between poliovirus proteins and the cell nucleus, despite that many processes in the poliovirus replication cycle occur in the cytoplasm.
脊髓灰质炎病毒基因组 RNA 的复制、蛋白翻译和病毒粒子组装都是在宿主细胞的细胞质中进行的。然而，这并不意味着脊髓灰质炎病毒感染与细胞核之间没有关系。在本研究中，荧光标记的重组脊髓灰质炎病毒 3CD 和 3C'蛋白在没有其他病毒蛋白的情况下主要在 Vero 细胞的细胞质中表达。然而，在脊髓灰质炎病毒感染后，许多这些蛋白重新分布到细胞核和细胞质中。一系列转染实验表明，脊髓灰质炎病毒 2A(pro) 负责将 3CD 和 3C'蛋白同样重新分配到细胞核。此外，缺乏蛋白酶活性的突变 2A(pro) 蛋白消除了这种效应。还发现脊髓灰质炎病毒 2A(pro) 蛋白与核孔复合物的核膜上的 Nup153 蛋白共定位。这些数据进一步证明了脊髓灰质炎病毒蛋白与细胞核之间存在内在相互作用，尽管脊髓灰质炎病毒复制周期中的许多过程都发生在细胞质中。
