水母发光蛋白检测到的刺激血小板无力症患者血小板中的钙变化。对ADP反应时聚集依赖性钙移动。

Aequorin-detected calcium changes in stimulated thrombasthenic platelets. Aggregation-dependent calcium movement in response to ADP.

作者信息

Lecompte T, Potevin F, Champeix P, Morel M C, Favier R, Hurtaud M F, Schlegel N, Samama M, Kaplan C

机构信息

Laboratoire d'Hématologie, Hôtel-Dieu, Paris, France.

出版信息

Thromb Res. 1990 Jun 15;58(6):561-70. doi: 10.1016/0049-3848(90)90302-s.

DOI:10.1016/0049-3848(90)90302-s

PMID:2117306

Abstract

Calcium changes in normal and thrombasthenic platelets were recorded using the PICA-apparatus. Aequorin was loaded in the presence of DMSO, EGTA and PGE1. Platelets of three patients with type I thrombasthenia stimulated with A-23, 187, thrombin, PMA in the presence 1 mM Ca++ and 1 mM Mg++ were able to normally raise their calcium concentrations. The maximal values could be found below the normal range with collagen, ADP and PAF-acether. Calcium mobilization from internal stores in response to thrombin was normal. There were two calcium peaks in normal platelets stimulated with ADP. The second one was suppressed by omitting fibrinogen, stirring, or by adding aspirin, and was absent in thrombasthenic platelets. Thus the GP IIb-IIIa complex is not a prerequisite for calcium fluxes but is involved, when weak agonists such ADP are used, through an aggregation-dependent reinforcement of platelet activation.

摘要

使用PICA仪器记录正常血小板和血小板无力症患者血小板中的钙变化。在二甲基亚砜、乙二醇双乙醚二胺四乙酸（EGTA）和前列腺素E1存在的情况下加载水母发光蛋白。三名I型血小板无力症患者的血小板在1 mM钙离子（Ca++）和1 mM镁离子（Mg++）存在的情况下，用A-23187、凝血酶、佛波酯（PMA）刺激时，能够正常提高其钙浓度。用胶原蛋白、二磷酸腺苷（ADP）和血小板活化因子（PAF-乙酰醚）刺激时，最大值可在正常范围以下找到。凝血酶刺激后从内部储存库动员钙的过程正常。用ADP刺激正常血小板时会出现两个钙峰。第二个钙峰可通过省略纤维蛋白原、搅拌或添加阿司匹林而被抑制，且在血小板无力症患者的血小板中不存在。因此，糖蛋白IIb-IIIa复合物不是钙通量的先决条件，但当使用ADP等弱激动剂时，它会通过血小板活化的聚集依赖性增强而参与其中。