Sun Xiu-juan, Yu Yan-ni, Xiao Yu-ming
Department of Pathology, Guiyang Medical College, Guiyang 550004, China.
Zhonghua Bing Li Xue Za Zhi. 2010 Oct;39(10):695-700.
To investigate mRNA and protein expressions of OPGL and M-CSF mRNA in bones of rats with experimental fluorosis induced by intake of fluoride in the drinking water, and to study the antagonizing effects of Danlan Xianpeng Liaofu capsules on the gene expression.
Totally 72 SD rats were randomly assorted into 6 groups including the control group, the fluoride group, the high-dosage (0.8 g/kg×d), mid-dosage (0.4 g/kg×d) and low dosage (0.2 g/kg×d) medication groups and the borax group (borax, 0.8 g/kg×d). The distribution of female and male rats in each group was divided up on a fifty-fifty basis. Except the control group, a NaF containing water (NaF 50 mg/L in concentration) was supplied as the drinking water for all the experimental rats in order to establish experimental fluorosis. The thickness and density of trabecula and the thickness of bone cortex were measured by light microscopy. The fluoride content in urine and bone were analyzed by using fluoride ion selective electrode method. Expressions of OPGL and M-CSF mRNA and protein were studied using RT-PCR and immuno-histochemistry, respectively.
(1) 10/12 of the experimental fluorosis rats developed dental fluorosis, and 2/12 of dental fluorosis rats occurred in the low-dosage medication group. Fluoride content in urine and bone of the fluorosis rats increased (P<0.05). (2) Compared with that of the control rats, the bone trabecular depth, cortical thickness and trabecular density in experimental fluorosis rats were remarkably reduced. (3) Compared with that of the control group, mRNA expression of both OPGL and M-CSF was increased in the fluoride group rats. The difference was statistically significant (P<0.05). (4) Compared with that of the fluoride group animals, the expression intensity of OPGL mRNA decreased in animals of the control group, the high, mid- and low- dosage medication groups and the borax group. Among them, except the low-dosage group, the difference between all the other groups and the fluoride group was statistically significant, respectively (P<0.05). There was also a decrease of M-CSF mRNA in all the 3 medication groups and the borax group animals in comparing with that of the fluoride group and the difference was also statistically significant (P<0.05), respectively. (5) Compared with that of the control group. There were an increase of OPGL and a decrease of M-CSF protein expression; and in addition, there were a decrease of OPGL and an increase of M-CSF protein expression in all 3 medication groups and the borax group in comparing with that of the fluoride group anima (P < 0.05).
Excessive fluoride induces an accelerated bone turnover and may promote the absorption activity of osteoclasts by increasing the expression of OPGL and M-CSF. Danlan Xianpeng Liaofu capsule may be capable of regulating bone remodeling through a down-regulation on OPGL and M-CSF expression.
探讨饮用含氟水诱导的实验性氟中毒大鼠骨骼中骨保护素配体（OPGL）和巨噬细胞集落刺激因子（M-CSF）mRNA及蛋白表达情况，并研究丹蓝仙鹏疗氟胶囊对基因表达的拮抗作用。
将72只SD大鼠随机分为6组，即对照组、氟中毒组、高剂量（0.8 g/kg×d）、中剂量（0.4 g/kg×d）和低剂量（0.2 g/kg×d）药物治疗组以及硼砂组（硼砂，0.8 g/kg×d）。每组雌雄大鼠分布比例均为50%。除对照组外，给所有实验大鼠饮用含氟化钠的水（浓度为50 mg/L的NaF）以建立实验性氟中毒模型。通过光学显微镜测量小梁厚度和密度以及骨皮质厚度。采用氟离子选择电极法分析尿液和骨骼中的氟含量。分别用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法研究OPGL和M-CSF mRNA及蛋白表达。
（1）12只实验性氟中毒大鼠中有10只出现氟斑牙，低剂量药物治疗组中有2只出现氟斑牙。氟中毒大鼠尿液和骨骼中的氟含量增加（P<0.05）。（2）与对照大鼠相比，实验性氟中毒大鼠的骨小梁深度、皮质厚度和小梁密度显著降低。（3）与对照组相比，氟中毒组大鼠OPGL和M-CSF的mRNA表达均增加。差异具有统计学意义（P<0.05）。（4）与氟中毒组动物相比，对照组、高、中、低剂量药物治疗组和硼砂组动物OPGL mRNA的表达强度降低。其中，除低剂量组外，其他各组与氟中毒组之间的差异均具有统计学意义（P<0.05）。与氟中毒组相比，3个药物治疗组和硼砂组动物M-CSF mRNA也均降低，差异也具有统计学意义（P<0.05）。（5）与对照组相比，OPGL蛋白表达增加，M-CSF蛋白表达降低；此外，与氟中毒组动物相比，3个药物治疗组和硼砂组OPGL蛋白表达降低，M-CSF蛋白表达增加（P<0.05）。
过量氟可导致骨转换加速，可能通过增加OPGL和M-CSF的表达促进破骨细胞的吸收活性。丹蓝仙鹏疗氟胶囊可能通过下调OPGL和M-CSF的表达来调节骨重塑。
