Department of Cell Biology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion, Chile.
FEBS Lett. 2011 Jan 21;585(2):390-6. doi: 10.1016/j.febslet.2010.12.020. Epub 2010 Dec 19.
In addition to its role as a strong antioxidant, vitamin C regulates the differentiation of several cell lineages. In vertebrate skeletal muscle, the vitamin C transporter SVCT2 is preferentially expressed in slow muscle fibers. To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. SVCT2 expression increases in cultures of both, slow and fast muscle-derived myoblasts, as they fuse to form mainly fast myotubes. Interestingly, we found that SVCT2 could be positively modulated by potassium-induced depolarization of myotubes. These findings suggest that SVCT2-mediated uptake of vitamin C could play diverse roles on skeletal muscle development and physiology.
除了作为一种强大的抗氧化剂外，维生素 C 还调节着几种细胞谱系的分化。在脊椎动物骨骼肌中，维生素 C 转运蛋白 SVCT2 优先在慢肌纤维中表达。为了深入了解细胞内维生素 C 对早期成肌作用的可能影响，我们研究了鸡胎生肌细胞培养物中 SVCT2 表达的调节。在慢肌和快肌衍生的成肌细胞融合形成主要为快肌管时，SVCT2 的表达在两种培养物中均增加。有趣的是，我们发现钾诱导的肌管去极化可正向调节 SVCT2。这些发现表明，SVCT2 介导的维生素 C 摄取可能在骨骼肌肉发育和生理学中发挥多种作用。
