Xia Li-ping, Li Ling-yun, Fei Xi-feng, Liang Zhong-qin
Yancheng Health Vocational and Technical College, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Dec;30(12):2649-51.
To study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA).
Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.
Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.
The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.
研究自噬在6-羟基多巴胺（6-OHDA）诱导的多巴胺能神经元死亡中的作用。
通过立体定向向单侧黑质致密部（SNpc）注射8μg 6-OHDA建立帕金森病（PD）大鼠模型。用透射电子显微镜（TEM）观察SNpc中的自噬体，并用免疫荧光（IF）测定法检测自噬相关蛋白LC3的表达。
在TEM下，6-OHDA注射后6-24小时在同侧SNpc中发现自噬体，这表明自噬被激活。IF测定显示，与对照组相比，6-OHDA损伤的TH阳性神经元中LC3表达显著增加。
自噬体的增加和自噬的激活可能在6-OHDA诱导的多巴胺能神经元死亡中起作用。
