De Lucca Anthony J, Boué Stephen M, Carter-Wientjes Carol H, Bland John M, Bhatnagar Deepak, Cleveland Thomas E
Southern Regional Research Center, USDA, ARS, New Orleans, LA 70124, USA.
Ann Agric Environ Med. 2010;17(2):301-8.
Toxigenic and atoxigenic strains of Aspergillus flavus were grown on potato dextrose agar (PDA) and wetted (23% moisture) sterile, cracked corn for 14 and 21 days, respectively. Volatile compounds produced by A. flavus, as well as those present in the PDA controls and sterile cracked maize, were collected using solid-phase micro-extraction (SPME) and identified by gas chromatography/mass spectrometry. Results show that growth substrate had a major impact on the number and type of volatiles detected. Growth on sterile cracked maize produced many more volatiles than did potato dextrose agar. There were also differences observed in the type of volatiles produced between toxigenic and non-toxigenic isolates, as well as between isolates of the same toxigenic grouping.
产毒和不产毒黄曲霉菌株分别在马铃薯葡萄糖琼脂（PDA）和湿润（含水量23%）的无菌破碎玉米粒上培养14天和21天。使用固相微萃取（SPME）收集黄曲霉产生的挥发性化合物，以及PDA对照和无菌破碎玉米中存在的挥发性化合物，并通过气相色谱/质谱进行鉴定。结果表明，生长基质对检测到的挥发性化合物的数量和类型有重大影响。在无菌破碎玉米粒上生长产生的挥发性化合物比在马铃薯葡萄糖琼脂上生长产生的要多得多。在产毒和非产毒分离株之间，以及同一产毒组的分离株之间，所产生的挥发性化合物类型也存在差异。
