局灶性脑缺血/再灌注时微血管内皮细胞ELAM-1和ICAM-1 mRNA表达的研究
[Study on expression of ELAM-1 and ICAM-1 mRNA on microvascular endothelial cells during focal cerebral ischemia/reperfusion].
作者信息
Wang Z X, Cai S P, Xu J
机构信息
Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 100853, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2001 Aug;17(3):236-9.
AIM
To evaluate the role of ELAM-1 and ICAM-1 in the course of inflammatory reactions during focal brain ischemia/reperfusion.
METHODS
The focal brain ischemia/reperfusion model is carried by occluding middle cerebral artery. The expression of ELAM-1 and ICAM-1 mRNA after ischemia/reperfusion was evaluated with RT-PCR.
RESULTS
No ELAM-1 and ICAM-1 mRNA were detected in the sham-operated cortex and only little in the nonischemic cortex. The expression of ELAM-1 and ICAM-1 mRNA were upregulated at 1 hour, peaked at 6 hour and 3 hour respectively and remained elevated for up to 48 hours after ischemia/reperfusion.
CONCLUSION
ELAM-1 and ICAM-1 participate in brain injury during focal ischemia/reperfusion and both of them play an important role in leukocyte infiltration into the ischemic tissues.
目的
评估ELAM-1和ICAM-1在局灶性脑缺血/再灌注炎症反应过程中的作用。
方法
通过阻断大脑中动脉建立局灶性脑缺血/再灌注模型。采用逆转录聚合酶链反应（RT-PCR）评估缺血/再灌注后ELAM-1和ICAM-1 mRNA的表达。
结果
在假手术组皮层未检测到ELAM-1和ICAM-1 mRNA，在非缺血皮层中也仅有少量表达。缺血/再灌注后1小时ELAM-1和ICAM-1 mRNA的表达上调，分别在6小时和3小时达到峰值，并在缺血/再灌注后长达48小时持续升高。
结论
ELAM-1和ICAM-1参与局灶性缺血/再灌注期间的脑损伤，且二者在白细胞浸润至缺血组织过程中均起重要作用。
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