Institute of Microelectronics, NCSR Demokritos, Aghia Paraskevi, Attiki 153 10, Greece.
Colloids Surf B Biointerfaces. 2011 Apr 1;83(2):270-6. doi: 10.1016/j.colsurfb.2010.11.031. Epub 2010 Dec 1.
Treatment of poly(dimethylsiloxane) (PDMS) surfaces with SF(6) plasma results in the creation of high-surface-area nanotextured surfaces that considerably favour protein adsorption with respect to untreated ones. In order to employ such nanotextured surfaces as substrates for microarrays to be created and analysed using standard instrumentation, we fabricated thin PDMS films on top of standard low-cost microscope glass slides. The properties of both untreated and plasma-treated PDMS-coated slides towards spotting of protein solutions were evaluated in terms of spot signal intensity and homogeneity as well as of spot shape and size. It was found that the plasma-treated PDMS-coated glass slides provided highly homogeneous spots (mean intra-spot variation 7.6%) with spot signal intensity 6-times higher than that obtained using the untreated ones. In addition, comparison with commercially available polystyrene and aminosilanized-glass microarray slides showed that the proposed slides provided 3-times higher spot signal intensity and 2-times lower intra-spot signal variation. In addition, the implementation of long-aged-after-plasma-treatment nanotextured PDMS-coated glass slides provided spots whose shape and size matched those of the spotting tip. As a consequence, denser arrays of variable spot shape can be created using SF(6) plasma-treated PDMS-coated slides instead of standard microarray slides opening new potentials for bioanalytical applications.
用 SF(6) 等离子体处理聚二甲基硅氧烷 (PDMS) 表面会产生具有高表面积的纳米纹理表面，与未处理的表面相比，这些表面非常有利于蛋白质的吸附。为了将这种纳米纹理表面用作微阵列的基底，以便使用标准仪器进行创建和分析，我们在标准低成本显微镜载玻片上制造了薄的 PDMS 薄膜。我们根据斑点信号强度和均匀性以及斑点形状和大小来评估未经处理和等离子体处理的 PDMS 涂层载玻片对蛋白质溶液点样的性能。结果发现，等离子体处理的 PDMS 涂层玻璃载玻片提供了高度均匀的斑点（平均斑点内变异性为 7.6%），其斑点信号强度比未处理的高 6 倍。此外，与市售的聚苯乙烯和氨基硅烷化玻璃微阵列载玻片的比较表明，所提出的载玻片提供了 3 倍高的斑点信号强度和 2 倍低的斑点内信号变异性。此外，经过长时间等离子体处理后的纳米纹理 PDMS 涂层玻璃载玻片的实施提供了形状和大小与点样尖端匹配的斑点。因此，使用 SF(6) 等离子体处理的 PDMS 涂层载玻片可以创建具有可变斑点形状的更密集的阵列，从而为生物分析应用开辟了新的潜力。
