Department of Inorganic Chemistry, Wrocław Medical University, Szewska 38, 50-139 Wrocław, Poland.
J Inorg Biochem. 2011 Feb;105(2):297-302. doi: 10.1016/j.jinorgbio.2010.11.011. Epub 2010 Nov 26.
The coordination properties of cyclic octapeptides with multi-His motif: c(His-Gly-His-Xaa-His-Gly-His-Xaa) where Xaa = Asp or Lys, were investigated. The binding abilities of this peptides towards Cu(II) ions were studied by using different analytic methods as: potentiometry, spectroscopy and mass spectrometry. The obtained results show that the studied peptides in physiological related pH prefer formation of the species with the {4N(Im)} binding mode. The efficiency of Cu(II) binding depends on additional side chain groups Asp or Lys. Additionally the analysis of results for His containing cyclopeptides with different numbers of amino acid residues in cyclopeptide ring e.g. four, eight shows that in higher pH in both cases the binding by four amide nitrogens is not observed in the case of α-amino acid peptides.
研究了具有多 His 基序的环状八肽（c（His-Gly-His-Xaa-His-Gly-His-Xaa），其中 Xaa = Asp 或 Lys）的配位性质。使用不同的分析方法（如电位法、光谱法和质谱法）研究了这些肽与 Cu(II)离子的结合能力。研究结果表明，在生理相关 pH 值下，研究的肽更倾向于形成具有 {4N(Im)} 结合模式的物种。Cu(II)结合的效率取决于额外的侧链基团 Asp 或 Lys。此外，对具有不同氨基酸残基数的环八肽的 His 进行分析，例如四肽和八肽，结果表明在较高 pH 值下，对于α-氨基酸肽，在两种情况下均未观察到通过四个酰胺氮的结合。
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